Challenges in the Development of In Vitro Screens for Assessing Non-CYP Mediated Metabolism
1 Jun 2014The aim of this study was to address the feasibility of developing in vitro metabolic screens for assessing the main non-CYP phase I enzymes responsible for metabolism of NCEs in human liver cytosol. Enzymes investigated include the molybdenum hydroxylases; aldehyde oxidase (AOX) and xanthine oxidase (XO), the alcohol and aldehyde dehydrogenases (ADH and ALDH), aldoketo reductases (AKR) and NADPH: quinone oxidoreductases (NQO).
Links
Tags
ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cytochrome P450 AssaysDrug MetabolismDrug metabolism is the enzymatic conversion within the body of pharmaceutical substances into other chemical compounds. The primary site for drug metabolism is the liver.