Cell painting high-content screening assay

Cell painting provides an image-based profiling tool amendable to the development of drug discovery assays and multiparameter screening campaigns. Cell painting assays can enable identification of hits in initial discovery screens and can provide mechanistic insights unavailable through traditional high-throughput assays. Thermo Fisher Scientific explores how the CellInsight™ CX7 LZR Pro instrument powered by HCS Studio™ 5.0 Cell Analysis Software and the Image-iT™ Cell Painting Kit help enable the development of next-generation drug discovery and safety mechanism of actions. They are preconfigured for multiparameter cell painting and the cell painting assay can be customized to accommodate a different range of targets or to accommodate a different type of cell. Cell painting using the CellInsight CX7 Pro series and HCS Studio 5.0 software allows the collection of all five channels of data required by the cell painting assay while using either software or laser autofocusing, to populate all image collection settings and analysis parameters.