Cell culture quality control during T cell expansion

3 Aug 2019

In this application note, Thermo Fisher Scientific describes a workflow that enables the isolation, activation, and expansion of robust T cells for downstream immuno-oncology applications. The Countess II FL Automated Cell Counter provides fast, accurate measurements of T cell viability and concentration.

Invitrogen™ Countess™ 3 FL Automated Cell Counter

Thermo Fisher Scientific

The Countess 3 FL Automated Cell Counter is both a brightfield and fluorescence cell counter that uses advanced machine-learning image analysis algorithms to deliver accurate cell counts and viability.  The fluorescence capabilities are user-determined with the ability to switch out light cubes and is useful for reporting and measuring fluorescent protein expression, apoptosis, and cell viability using fluorescent reagents.

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Cell culture quality control during T cell expansion