Brief HPLC analysis of hydrocortisone in accordance with the United States Pharmacopeia
10 Mar 2023Hydrocortisone is the pharmaceutical term for cortisol used in oral administration, intravenous injection, or topical application. It is often used as an immunosuppressive drug, given by injection, in the treatment of severe allergic reactions, such as anaphylaxis and angioedema. The United States Pharmacopeia (USP) provides monographs for the identification and impurity testing of the drug substances. This application note describes the use of PerkinElmer Epic™ C18, Epic™ C18 SD (highly base deactivated high carbon super dense phase), and Epic™ Polar (150 x 4.6 mm, 5 µm) columns, coupled with the PerkinElmer LC 300 liquid chromatography system and SimplicityChrom™ Chromatography Data System (CDS), for the assay analysis of hydrocortisone.
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Epic C18 Liquid Chromatography Columns
PerkinElmerThe Epic™ line of LC columns is based on high density monomerically bonded phases produced through a proprietary bonding process. Epic HPLC columns are compatible with a wide range of organic modifiers and buffers and stable over a wide pH range. All Epic products use an ultra-high purity metal free silica and undergo strict quality control testing. For flexibility, we offer a wide range of column sizes as well being fully scalable from analytical to preparative dimensions.