LC 300™ HPLC & UHPLC System
Flexibility, performance and efficiency with the PerkinElmer LC 300 HPLC and UHPLC System
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Great results
Sample analysis and purification
Very good in result and sophisticated
Review Date: 23 Jun 2023 | PerkinElmer
Reliable and robust. Consistently producing good results.
Sample Analysis of PAHs
The elegantly designed machine is both easy on the eyes as it is reliable in producing good results. Capable of achieving high quality data and reproducible results even by novice experimenters such as undergraduate students. Relatively easy to use but since it has been installed and used, it has not needed any repairs or maintenance thus far other than the regular tweaks for daily operations. So far so excellent. A good product thus far. Thank you Perkin Elmer.
Review Date: 19 Jun 2023 | PerkinElmer
Only LC-300 can manage to exceed your most demanding expectations. Solo LC-300 puede lograr superar sus expectativas más exigentes.
Pharma
This is a good product, robust, with very friendly software, meets demanding specifications and is available in different configurations. Este es un buen producto, robusto, con un software muy amigable, cumple especificaciones exigentes y esta disponible en diferentes configuraciones.
Review Date: 17 May 2023 | PerkinElmer
Great analytical results almost everytime.
Sample analysis
One of the best modern instruments in our Analytical Chemistry Lab.
Review Date: 27 Mar 2023 | PerkinElmer
I will never recommend this instrument to anyone.
Analyzed the concentrations of pesticides in liquid and soil
We received this instrument past more than three month from the appointed day due to the delayed assmebly. After the arrival of the instrument we couldn't use it for one month due to a communication problem between the instrument and PC. The problem was resolved by exchange one of its modules. When LC300 is compared with our previous HPLC, LC10 of Shimadzu, it produces larger noise of baseline and the retention times of analytes significantly change thus makes a reliable analysis difficult. In addition the analysis results can be compared only in the single experimental run (we can not compare the results in different runs). The analysis results is not stored in a file but stored only in the program. Therefore when we must format the PC we cannot transfer the result to other storage media such as a USB memory (we can save the results as a PDF file containing the HPLC chromatograms and results tables).
Review Date: 29 May 2022 | PerkinElmer
Excellent results, just the one every lab recent compliance looks for
Life sciences and pharmaceutical analysis
Applicability is excellent and one of state of Art experience to work with. Extremely precise and sensitive with research exploration and inspiration to enhance research vigor
Review Date: 24 Jan 2022 | PerkinElmer
The PerkinElmer LC 300 HPLC & UHPLC System has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the highly intuitive and powerful SimplicityChrom™ Chromatography Data System (CDS) Software, PerkinElmer LC 300 HPLC & UHPLC System are the ideal solution for labs seeking to maximize their liquid chromatography experience.
The LC 300 HPLC & UHPLC System offers superior performance and flexibility throughout its intelligent design:
- Robust and precise autosampler with integrated column oven and preheater, as well as a high visibility color LCD display showing full instrument system status, current queue and sample progress.
- Optional external Peltier column oven for ultra-precise heating and cooling, for sample integrity and exceptionally accurate results.
- Patented Intermediate Loop Decompression (ILD™) valve ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.
- Automated, onboard sample dilution and derivatization capabilities simplify the front-end analytical processes, while also ensuring reproducibility and precision.
- Powerful 10/18k psi pump for ultra-precise UHPLC analyses, delivering repeatable solvent delivery at a wide range of flow rates and pressures.
- Automatic priming design allows the system to achieve full self-priming capabilities, automating the otherwise cumbersome priming process utilized in many other systems.
- Built-in solvent degassing technology simplifies the analytical process, and obviates the need for a separate, external degassing module.
- Portfolio of five available detectors, which can be used individually or in sequence. Photodiode array (PDA) Multiwavelength UV/Vis (MWD) Single wavelength UV/Vis Refractive index (RI) Fluorescence (FL).
- Efficient and powerful instrument control with SimplicityChrom CDS System, allowing users to create custom workflows and experiences, all while achieving compliance with 21 CFR Part 11 requirements.
Brochures
Explore the LC 300 HPLC and UHPLC Systems
Laboratories face growing demands for increased throughput, improved margins, and more efficient processes. In this interactive brochure, learn more about the PerkinElmer LC 300 HPLC and UHPLC Systems, with features and benefits such as five available detectors, ultraprecise gradient flows, low dispersion, and new Simplicity™Chrom CDS software. The LC 300 systems provide improved workflows, throughput, and usability.
Brief HPLC analysis of hydrocortisone in accordance with the United States Pharmacopeia
Hydrocortisone is the pharmaceutical term for cortisol used in oral administration, intravenous injection, or topical application. It is often used as an immunosuppressive drug, given by injection, in the treatment of severe allergic reactions, such as anaphylaxis and angioedema. The United States Pharmacopeia (USP) provides monographs for the identification and impurity testing of the drug substances. This application note describes the use of PerkinElmer Epic™ C18, Epic™ C18 SD (highly base deactivated high carbon super dense phase), and Epic™ Polar (150 x 4.6 mm, 5 µm) columns, coupled with the PerkinElmer LC 300 liquid chromatography system and SimplicityChrom™ Chromatography Data System (CDS), for the assay analysis of hydrocortisone.
Analysis of saccharides in high fructose corn syrup by HPLC with refractive index detection
There are many uses for HPLC as a monitoring and quality control tool in the various wet corn milling processes. One example is high fructose corn syrup (HFCS), an ingredient often used as a substitute for sucrose in a wide variety of products which have high demands for production efficiency and product consistency. In this application note, learn more about how regulatory compliance and product consistency are ensured. Manufacturers need to implement robust analytical methods to determine the saccharide composition of high fructose corn syrups. One widely used industry method is SACCH.03 from the Analytical Methods of the Member Companies of the Corn Refiners Association (CRA) which relies on HPLC coupled to refractive index (RI) detection for the measurement of saccharide content.
HPLC analysis of phenolic antioxidants in edible fats and oils supporting food quality control and label transparency
Fats and oils are used in cooking as a frying medium or as an ingredient in baking. Autooxidation of lipids in the fats and oils proceeds as a free-radical chain reaction that leads to rancidity causing foul flavors and smells. This application note from PerkinElmer presents a fast, robust, and sensitive liquid chromatographic method for the analysis of ten commonly used phenolic antioxidants, and builds upon prior work.
HPLC: determination of urea content in urea-based fertilizers in accordance with AOAC official method 2003.14
Fertilizers are agrochemicals that are added to soil or land to increase its fertility. They can be broken down into two major categories: organic (natural, carbon containing) or synthetic. Nitrogen, phosphorus, and potassium are the primary nutrients in fertilizers. This application note demonstrates the suitability of the PerkinElmer LC 300 HPLC System to determine free urea in urea-based fertilizers via the Association of Official Analytical Chemists (AOAC) Official Method 2003.14 through the analysis of five commercially available fertilizer products.
