Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing

Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing

8 Jun 2015

This application note describes the use of the Bioruptor® NGS to prepare the samples in the sequencing of two bacterial genomes with dissimilar GC characteristics - Escherichia coli and Staphylococcus epidermis.

Bioruptor® Plus

Diagenode

The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. An excellent device for shearing chromatin or DNA, cell and tissue disruption and many other applications. Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. The Bioruptor® Sonication System uses a sonication bath to generate indirect sonication waves, which emanate from an ultrasound element below the water tank. Because the system is gentler than other sonicators, the Bioruptor® produces better and more consistent results than with harsher sonication methods. Up to 12 closed tubes can be sonicated in parallel and the continuous rotation of tubes allows even distribution of the energy for efficient sonication. The Bioruptor® enables automation of sonication, guaranteeing higher reproducibility of results. Features: Digital timer Metal soundproof box Overheat shutdown protection (this feature is not available in the US) Machine lifetime control Applications: Chromatin Shearing DNA Shearing Cell & Tissue disruption

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Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing