ResourceLife Sciences
Biological advances using the protein footprinting method covalent protein painting
24 Nov 2025This application note introduces covalent protein footprinting (CPP), a mass spectrometry-based method for analyzing protein conformation and surface accessibility. Using isotope-labeled dimethyl tagging, the approach enables quantitative measurement of structural changes in proteins across complex biological samples.
CPP has been applied to study protein structural changes in neurodegenerative disease, cancer cell lines, and in vivo models. By revealing conformational alterations that can occur before expression changes, the method provides valuable insights for understanding disease mechanisms and identifying potential biomarkers.
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- Resource type: Application notes
- Page count: 2
- Read time: 3 mins
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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ProteomicsProteomics refers to the analysis of proteins through a variety of different experiments in order to understand structure and functionality. Typical techniques include protein purification and mass spectrometry.