Best Workflow Practices for ChIP-seq Analysis with Small Samples

The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microChIP-seq assay has been validated with the new generation IP-Star® Compact Automated Workstation. Furthermore Diagenode's high quality ChIP-seq grade antibodies have been used. Features: Revolutionary: Only 10.000 cells needed for complete ChIP-seq procedure Reliable and accurate sequencing library preparation from just picogram inputs Validated on the IP-Star® Compact Automated Platform

The easiest NGS Library preparation you will ever find! The MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the True MicrochIP kit, it allows for performing ChIP-seq on as few as 10,000 cells. Studies showed a perfect match between the top 40% of peaks and the reference ChIP-seq dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset. With less input, fewer steps, fewer supplies, and faster time to results, the MicroPlex kit is the library preparation solution of choice. MicroPlex Library Preparation™ Kit Features: Simplified protocol: single tube, 3-step protocol without any intermediate purifications 3 times faster than TruSeq™ - only 2 hours for entire protocol DNA sample inputs from only 50 pg Specifically optimized for ChIP-seq Fewer PCR cycles: less bias Indexing options, easily automated Proven accuracy and reproducibility  Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq samples and are optimized to generate DNA libraries with high molecular complexity from the lowest input amounts – down to 50 pg. The kit MicroPlex v3 includes all buffers and enzymes necessary for the library preparation. For flexibility of the choice different formats of compatible dual indexes are available separately.  

The Bioruptor® Pico is the new next-generation all-in-one shearing device, highly optimized for DNA shearing for next-generation sequencing, chromatin shearing for ChIP-seq applications, RNA shearing, protein extraction, and cell/tissue lysis. The Pico is the shearing device of choice for sequencing applications, providing optimal yields, lengths, and consistency and excels in shearing different fragment size ranges, which are frequently required for downstream applications (e.g. bridge amplification) for sequencing. Moreover, the Pico provides flexibility in other applications saving the need to buy additional equipment. Unlike competing systems, the Pico has high throughput capabilities and can process up to 12 samples at once. The only ultrasonicator you’ll ever need, optimized for: Next-Generation Sequencing (5 - 100 µl) Chromatin shearing (10 µl - 2 ml) RNA shearing Protein extraction Tissue and cell lysis Simultaneous sonication of 12 samples Small and light