ResourceLife Sciences
Baculovirus Titration with the Accuri C6 Flow Cytometer
2 Sept 2010The baculovirus expression vector system (BEVS) is increasingly popular among researchers as a tool for generating large amounts of recombinant protein in insect cells, which post-translationally process proteins in a manner similar to mammalian cells. This technical note demonstrates flow cytometry as a fast, accurate, and often less expensive, more efficient method to determine baculovirus titers.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.