Background-Free Analysis of Mouse TILs from Solid Tumors

Syngeneic mouse tumor models represent the gold standard to analyze effects of immunotherapy, as they possess a fully competent immune repertoire. Such analyses routinely rely on the characterization of tumor-infiltrating leukocytes (TILs) and their phenotype. However, the amount and composition of TILs is highly variable and often very low, complicating the flow cytometric analysis of individual subpopulations.