Automation of Luminex® NxTAG® Respiratory Pathogen Panel

20 Aug 2017

The objective of this study was to evaluate the Hamilton® Microlab STAR™ platform’s ability to automate simultaneous extraction of total nucleic acid from up to 96 contrived samples with subsequent addition of the extracted material to the NxTAG RPP reaction vessels. The Luminex NxTAG® Respiratory Pathogen Panel is a qualitative nucleic acid multiplex test that provides simultaneous detection and identification of 19 viruses and 3 atypical bacteria associated with respiratory tract infections.

Microlab STAR

Hamilton

Experience Automation Your Way. The Microlab STAR Line automates liquid handling tasks from simple to complex with exceptional precision and flexibility so that you can increase throughput, reduce errors, and gain confidence in results.

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Maxwell® 16 LEV RNA FFPE Purification Kit

Promega Corp.

  The Maxwell® 16 LEV RNA FFPE Purification Kit is designed to extract RNA rapidly from FFPE samples using the Maxwell® 16 Instrument (Cat.# AS2000 and AS3000).   The FFPE kit is specifically designed for optimal purification of RNA from 5μm thin sections of formalin-fixed, paraffin-embedded (FFPE) tissue samples. The Maxwell® 16 LEV RNA FFPE Purification Kit is optimized to yield a final RNA concentration that maximizes RNA yield, eliminating the need to concentrate the extract prior to amplification. Lysates are placed directly into the cartridges, and RNA ready for amplification is obtained in approximately 60 minutes (after proteinase K digest). This kit does not use hazardous xylene, thereby providing a much safer method than many competitive kits. Additionally, quality testing demonstrates virtually no PCR inhibitors in purified samples. Features & Benefits: Automated Platform for Extracting RNA from FFPE Mammalian Tissues: Provides an alternative to manual competitive offerings. Walkaway Automated Extraction from up to 16 Samples in a Single Run: Provides superior ease of use over competition. Provides High Yield, Pure and Amplifiable RNA from FFPE Mammalian Tissue Samples: Minimizes sample waste and reruns. Simple Method with No Organic Solvents for Deparaffinization: No exposure to toxic organic reagents to deparaffinize the FFPE tissue sample prior to RNA purification. Decreased Processing Time: Protocol can be completed within a single 8-hour shift. Applications: Automated RNA purification from FFPE samples.

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Maxwell® 16 LEV simplyRNA Purification Kits

Promega Corp.

  The Maxwell® 16 LEV simplyRNA Kits are for use with the Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor.   This RNA purification procedure is a simple method with minimal lysate handling before automated purification on the Maxwell® 16 Instrument. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. The kit provides the reagents for processing the samples and uses prefilled cartridges for purification, maximizing simplicity and convenience. Maxwell® simplyRNA Kit Features: Simple Preprocessing Steps; No Centrifugation: Save time and minimize errors. Automated RNA Extraction: Consistent results and sufficient yield for downstream assays. Includes All Reagents and Consumables, including DNase and All Plastics: No hidden costs plus ease of ordering. Use Fresh or Frozen Cells or Tissue: Versatile protocol. Applications: RNA extraction used in many areas of research including gene expression and translation.

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