Automated Sample Preparation for the Monitoring of Ethanol Metabolites in Urine by LC-MS/MS
Automated Sample Preparation for the Monitoring of Ethanol Metabolites in Urine by LC-MS/MS
21 Sept 2015Ethylglucuronide (EtG) and Ethylsulphate (EtS) are stable Phase II metabolites of ethanol, which can be detected in urine until several days after alcoholic beverage consumption. This application note describes an LC-MS/MS method for the analysis of EtG and EtS in urine employing a simple, automated sample preparation methodology using a liquid handling-based automation system. The Biomek 4000 Workstation was used to automate the sample processing for the forensic screening of EtG and EtS on the SCIEX Triple Quad™ 4500 LC/MS/MS system.
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