Automated parallel derivatization strategy with broad metabolite coverage coupled to SWATH/MS data acquisition for qualitative and quantitative analysis

Explore an automated parallel derivatization strategy with Dansyl-Cl and Dansyl-N2H3 for broad metabolite coverage in LC-MS workflows. By enhancing retention time, signal-to-noise ratio, and reaction control through on-line SPE fractionation, the method improves qualitative and quantitative metabolomics. Using labeled (13C) derivatization agents with SWATH/MS data acquisition enables precise metabolite analysis, addressing limitations in polar metabolite detection and quantification.