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Automated, High-Throughput LC-MS/MS Workflow for the Analysis of 25-Hydroxyvitamin D2/3 and 3-Epi-25-Hydroxyvitamin D3

10 Mar 2015

In this application note, an automated, high-throughput LC-MS/MS workflow was developed for the analysis of 25-hydroxyvitamin D2/3 and 3-epi-25-hydroxyvitamin D3 in human serum for research laboratories.

Thermo Scientific Versette

Thermo Fisher Scientific

The Thermo Scientific™ Versette is a compact automated liquid handler that offers 96- or 384-channel pipetting heads and user friendly programming options. Its reliable performance and compact size make it a flexible and convenient liquid handling solution for many kinds of laboratories. Versette is designed to perform a variety of liquid handling tasks for a wide range of applications including high-throughput immunoaffinity sample preparation with Mass Spectrometric Immunoassay (MSIA*) pipette tips. It also supports 96/384 plate replication, plate stamping and serial dilution procedures.Key Features: 96-and 384-channel pipetting heads Handle a total volume range of 0.5-300 µl All pipetting heads include RFID tags for self-identify, and utilize Thermo Scientific D.A.R.T.s tips Compact Size The six position stage uses a unique dual-level design to minimise space requirements Fits on standard benches and biological hoods User-friendly programming options: Simple pipetting procedures can be created using visual on-board graphical display For more complex protocols and advanced editing Thermo Scientific ControlMate software is available

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Automated, High-Throughput LC-MS/MS Workflow for the Analysis of 25-Hydroxyvitamin D2/3 and 3-Epi-25-Hydroxyvitamin D3