Automated ChIP with the SX-8G IP-Star®
Automated ChIP with the SX-8G IP-Star®
26 Apr 2017This application note demonstrates the automation of ChIP using the SX-8G IP-Star Automated System, allowing the entire ChIP procedure to be performed in a single day. Chromatin Immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell. It aims to determine whether specific proteins are associated with specific genomic regions, such as transcription factors on promoters or other DNA binding sites, and possibly defining cistromes. ChIP also aims to determine the specific location in the genome that various histone modifications are associated with, indicating the target of the histone modifiers.
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SX-8G IP-Star® Compact Automated System
DiagenodeChromatin immunoprecipitation and DNA methylation assays followed by next generation sequencing is a powerful technique for generating genome-wide profiles of epigenetic modifications and transcription factor binding sites. The SX-8G IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. Samples generated on the system are compatible with popular downstream applications such as QPCR, microarrays and next generation sequencing platforms. In addition to automated ChIP and DNA methylation protocols, this system contains a wide variety of protocols including next generation sequencing library preparation and sample purification. Easy-to-use user interface for protocol optimization Magnetic-bead technology for great reproducibility Reduced "hand on time" to just only 15 minutes Easy to use bench-top liquid handling system Process up to 16 samples in parallel True "walk-away" automation Flexibility to adapt your experimental condition