Analytical performance of nucleic acid microvolume quantification using the Epoch Spectrophotometer System

Nucleic acids such as DNA and RNA are derived from a variety of biological sources for potential use in downstream applications such as sequencing and gene expression analysis. This application note demonstrates the analytical performance of the Agilent BioTek Epoch spectrophotometer system and especially the Agilent BioTek Take3 multivolume plate with regard to linear dynamic range, limit of detection, precision, and accuracy using microvolume samples to verify its broad applicability to nucleic acid quantification. Comparative data were derived on an Epoch spectrophotometer system and a NanoDrop; performance was shown to be equivalent.