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Analysis of Vitamin D in Milk and Infant Formula using UHPLC/MS/MS per AOAC Method 2011.11

Analysis of Vitamin D in Milk and Infant Formula using UHPLC/MS/MS per AOAC Method 2011.11

24 Nov 2015

Fortification of milk, dairy products, infant and adult nutritionals with vitamin D is required in many countries in order to provide additional dietary vitamin D. A method for the determination of nutritionally important vitamin D forms is presented here. Analysis is performed as standards, and extracted from milk and infant formula after saponification following AOAC method 2011.11 using our Titan™ C18 UHPLC column and MS/MS with APCI detection.

Titan™ C18 UHPLC Columns, 1.9 μm

Sigma-Aldrich Supelco

Titan UHPLC columns, based on 1.9 µm totally porous monodisperse silica, outperform other UHPLC columns. These UHPLC columns provide the performance scientists expect (typically > 250,000 N/m), but at a greatly reduced cost. With the narrowest particle size distribution of all available sub-2 µm porous particles, Titan columns are the outcome of the highly efficient patent pending Ecoporous™ silica manufacturing process that delivers high-performance silica at a low cost. Read about how this product was used to analyze Riboflavin and impurities here and used in purification methods here. Features & Benefits: • Excellent Performance • Extremely Narrow Particle Size Distribution • Great Value

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