ResourceSpectroscopy

An Alternative to the USP Method for the Analysis of Riboflavin (Vitamin B2) and Impurities

An Alternative to the USP Method for the Analysis of Riboflavin (Vitamin B2) and Impurities

18 Jan 2016

The method reported here provides a viable alternative to the cumbersome USP and EP methods for the analysis of Rioboflavin (Vitamin B2). The combination of the Titan™ C18 column and rapid acetonitrile gradient provided excellent resolution and short analysis time under conditions compatible with both MS and UV detection.

Titan™ C18 UHPLC Columns, 1.9 μm

Sigma-Aldrich Supelco

Titan UHPLC columns, based on 1.9 µm totally porous monodisperse silica, outperform other UHPLC columns. These UHPLC columns provide the performance scientists expect (typically > 250,000 N/m), but at a greatly reduced cost. With the narrowest particle size distribution of all available sub-2 µm porous particles, Titan columns are the outcome of the highly efficient patent pending Ecoporous™ silica manufacturing process that delivers high-performance silica at a low cost. Read about how this product was used to analyze Riboflavin and impurities here and used in purification methods here. Features & Benefits: • Excellent Performance • Extremely Narrow Particle Size Distribution • Great Value

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