Amplification of β-globin, K-ras 12, p53 (exon 5, exon 6) Genes from 10-Year-Old Paraffin Sections

This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products. Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for intercalator-based qPCR. Additionally, SYBR Premix Ex Taq II (Tli RNase H Plus) is supplied at a 2X concentration, enabling easy reaction assembly. The premix also contains heat-resistant Tli RNase H Plus, which effectively degrades residual mRNA found in the input cDNA, thereby minimizing qPCR inhibition as a result of residual mRNA presence.Takara SYBR Green Master Mix products contain TaKaRa Ex Taq HS DNA Polymerase, a hot start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq antibody allows highly reproducible and reliable qPCR. SYBR Premix Ex Taq II (Tli RNase H Plus) also enables high reaction specificity and superior performance with GC-rich templates.Features: High specificity minimizes primer-dimer formation and nonspecific amplification Excels with GC-rich targets 2X master mix provides convenience High sensitivity: detects fewer than 100 copies Compatible with all commonly used qPCR instruments Includes SYBR Green Master Mix for intercalator-based qPCR Tli RNase H Plus reduces inhibition from mRNA/cDNA hybrids in qPCR Eliminates need for separate RNase H digestion step when using low RNase H RTs Applications: Gene expression analysis qPCR Genotyping Suppression of primer-dimers or off-target products