ResourceLife Sciences
Achieving Similar Quality Results with Lower Amounts of Protein in Corning® Epic® Biochemical Assays
21 Jan 2015For many laboratories, procuring, manufacturing, or purifying large quantities of a purified protein to perform a high throughput label-free biochemical assay can be problematic. In this study, a strategy was developed to reduce the amount of protein required while maintaining an immobilization level that provides robust results in the Epic biochemical assay described. Proteins with a wide range of pI values were included in this study to demonstrate the versatility of this approach.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.