Accurate and Precise GFP Quantification with DeNovix Fluorometer
31 Oct 2017Green fluorescent protein (GFP) is widely used in molecular and cell biology as a reporter gene for expression of proteins. GFP is an intrinsically fluorescent protein that is easily detected using a fluorometer or fluorescence microscope. This application note details a method of quantifying the GFP fluorophore using the DeNovix QFX Fluorometer.
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QFX Fluorometer
DeNovixThe DeNovix QFX Fluorometer offers the widest dynamic range, greatest sensitivity, and ground-breaking ease of use. The QFX includes four excitation sources and requires as little as 1 µL of sample. The system is compatible with DeNovix dsDNA and RNA Fluorescence Quantification assays, commonly used Thermo Fisher Qubit™ assays, or user-configured custom assays.
DS-11 Series Spectrophotometer / Fluorometer
DeNovixDeNovix takes spectrophotometry to a whole new level of performance with the DS-11 Series. This easy-to-use, compact spectrophotometer delivers full spectrum UV-Vis analysis and fluorescence capability, making it ideal for rapid nucleic acid and protein quality control.
FX Fluorometer Module from DeNovix
DeNovixInstantly add the power of fluorescence detection to DeNovix spectrophotometers. Simply plug in this USB-powered accessory to immediately enable DeNovix fluorometric EasyApps™ software. These easy-to-use apps are pre-configured for use with a wide choice of commercially available assays and offer the flexibility to customize new assays and fluorophores.