ResourceLife Sciences
Accelerating IVT workflows with PCR-generated DNA templates
6 Oct 2026This application note evaluates PCR-generated DNA templates as a streamlined alternative to traditional plasmid amplification for in vitro transcription (IVT) workflows. Learn how PCR-based template generation eliminates bacterial cloning and culture steps while producing high-quality DNA templates for mRNA synthesis. Discover comparative data showing equivalent RNA yield, RNA integrity, double-stranded RNA by-product levels and protein expression performance between PCR-generated and plasmid-derived templates, demonstrating how PCR workflows can accelerate mRNA development, rapid prototyping and high-throughput IVT applications without compromising quality.
Resource details:
Resource type: Application note
Page count: 4
Read time: 6 mins
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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.