A Unique Sample Preparation Method for the Extraction of Ibuprofen from Urine for LC-MSMS Quantification
17 Feb 2015The AC Extraction Plate is a 96-well, deep-well plate providing a straightforward workflow that is reproducible and cost-effective. There are fewer steps compared to most commonly used sample preparation methods, and these steps are more easily implemented into automated workflows. This application note describes the extraction of ibuprofen from pig urine using a simple sample preparation workflow based on the AC Extraction Plate, and subsequent analysis by LC-MSMS.
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AC Extraction Plate™
TecanThe Tecan AC Extraction Plate* with TICE™ (Tecan Immobilized Coating Extraction) technology revolutionizes your sample preparation routine. The AC Extraction Plate is a 96-well, deep-well microplate, with each well having a proprietary TICE™ coating. TICE is a material with distinct absorptive properties, and this coating efficiently extracts specific small molecule analytes from aqueous solutions, releasing them when in contact with a suitable organic solvent. A simple pipette and shake sequence, with no filtration, centrifugation or solvent evaporation, is all that is required. The AC Extraction Plate is easily integrated into automated processes, making it a perfect match with Tecan’s Freedom EVO® liquid handling platform. AC Extraction Plate™ Features: Cost-effective – Simple pipette and shake workflow, without labor intensive centrifugation, filtration and solvent evaporation steps. Convenient and fast – Just unpack and start, no conditioning or other preparations needed. Highly reproducible – Pipette and shake routines are easily standardized and automated on a liquid handling platform, eliminating random errors and increasing consistency of results. * For research use only – not for clinical diagnostic applications