A reliable and simple FIA-MS/MS method for the quantitation of amino acids and acylcarnitines in dried blood spots

10 Nov 2023

This application note from Thermo Fisher Scientific details a FIA-MS/MS method using the Thermo Scientific™ Vanquish™ MD HPLC and Thermo Scientific™ TSQ Quantis™ MD MS. The method, validated with ClinSpot™ LC-MS/MS Complete Kits, enables the detection of 12 amino acids and 13 acylcarnitines from dried blood spot samples. It fulfills the requirement for semi-quantitative determination of these compounds, crucial for newborn screening.

Thermo Scientific™ Vanquish™ MD HPLC System

Thermo Fisher Scientific

Develop in vitro diagnostic tests with superior resolution. The Thermo Scientific™ Vanquish™ MD HPLC is the ideal chromatographic separations system for laboratories whose analyte resolution is more critical than sample throughput and batch turnaround time. The small, powerful single channel Vanquish MD HPLC meets analytical needs, as well as space and budget limitations.

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A reliable and simple FIA-MS/MS method for the quantitation of amino acids and acylcarnitines in dried blood spots