A novel IHC technology for tissue cross reactivity screening of humanized antibodies in human tissue

This scientific poster introduces Vector Human-on-Human Detection, a novel immunohistochemistry technology designed for tissue cross-reactivity screening of humanized therapeutic antibodies in human tissues. The method overcomes challenges caused by endogenous human immunoglobulins that typically generate high background signals in standard IHC. Using a proprietary quenching reagent and HRP-based detection, the approach enables sensitive and specific detection of therapeutic antibodies in frozen and FFPE tissues without requiring antibody modification. The technology demonstrates strong signal-to-noise ratios and reliable detection across multiple therapeutic antibodies, supporting improved safety assessment during preclinical antibody development.