A New Quantitative Microplate-Based Multiplex Technology

The Mosaic ELISA Human Growth Factor Panel 1 is an excellent tool for the detection of 6 different growth factors in the same sample. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments. Growth factor is a term used to describe a broad range of structurally diverse molecular families and individual proteins best known for their ability to enhance cell proliferation and growth. They have an array of putative functions during development including regulating tissue morphogenesis, angiogenesis, cell differentiation, and neurite outgrowth. They also play important roles in the maintenance of tissue homeostasis and wound healing in the adult. Their activities are mediated via binding to transmembrane receptors that often contain cytoplasmic tyrosine kinase domains. Product Features   •  96-well microplate-based assay   •  Simultaneously detects 6 analytes   •  Validated for cell culture supernates, serum, and plasma   •  Requires small sample volume (13-50 µL/well*)   •  3.5 hour assay time   •  Amenable to high-throughput   •  240 data points per microplate (40 samples plus a standard curve in duplicate) *Serum/plasma/platelet-poor plasma samples require a 4-fold dilution. Kit Contents   •  Each well is spotted with 6 capture antibodies   •  Each well is spotted with 1 reference spot   •  Cocktail of Purified Protein Standards   •  Detection Antibodies   •  Diluent & Wash Buffers   •  Substrate Reagents   •  Plate Covers

The Mosaic ELISA Human Cytokine Panel 1 is an excellent tool for the detection of 8 different cytokines in the same sample. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments. Cytokines are pleiotropic extracellular signaling molecules that influence myriad physiological and pathological processes. Released from a variety of cells, their actions affect cellular growth, differentiation, gene expression, migration, immunity, and inflammation. In most biological processes, multiple cytokines operate in a large network where the action of one cytokine is regulated by the presence or absence of other cytokines. Studying the underlying signaling cascades is further complicated by the fact that many cytokines modulate the production of other cytokines. Product Features   •  96-well microplate-based assay   •  Simultaneously detects 8 analytes   •  Validated for cell culture supernates, serum, and plasma   •  Requires small sample size (25 µL*)   • Takes 4.5 hours to run   •  Amenable to high-throughput   •  320 data points per microplate (40 samples in duplicate plus 2 standard curves) *Serum/plasma/platelet-poor plasma samples require a 2-fold dilution. Kit Contents   •  Each well is spotted with 8 capture antibodies   •  Each well is spotted with 1 reference spot   •  Cocktail of Purified Protein Standards   •  Detection Antibodies   •  Diluent & Wash Buffers   •  Substrate Reagents   •  Plate Covers