A fast and simple workflow for mAb post-translational modifications study using LCMS-9030 Q-TOF

During drug development, it is crucial to closely monitor the changes in the post-translational modification (PTM) amino acid relative site abundance percentage values, such as methionine (M) oxidation or asparagine (N) deamidation, as it can impact the efficacy, stability and immunogenicity of monoclonal antibodies (mAbs).

Explore a simple and straightforward workflow to quantify the amino acid site relative abundance using peptide mapping analysis. Discover how a Shimadzu LCMS-9030 QTOF mass spectrometer coupled with Protein Metrics software provides high mass resolution to accurately identify, separate, and quantify the modified peptides with small mass shift differences. The use of peptide mapping analysis for PTM study can provide valuable insights into the mAbs characteristics to support early to late-stage drug development.