Fluorescence Enhancement Reagents
The Qubit dsDNA broad range assay kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl.
Simply add your sample (between 1 µL and 20 µL) to the ready-to-use solution and read the dsDNA concentration (10 pg/µL to 100 ng/µL) on the Qubit Fluorometer
Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation.
Dextran Sulfate (MW 500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) solution.
The Qubit RNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of high-abundance RNA samples.
The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples.
Quickly determine quality and integrity of an RNA sample. The Qubit RNA IQ Assay Kit assesses RNA quality at a lower cost and with a simple workflow.
Simply add your sample (between 1 µL and 20 µL) to the ready-to-use solution and read the dsDNA concentration using a fluorescent-based microplate reader.
The Qubit RNA XR (Extended Range) Assay Kit, designed for use with the Qubit 4 and Qubit Flex fluorometers, provides an accurate and selective method for the quantitation of high-abundance RNA samples.
Sapphire-II™ and Emerald-II™ chemiluminescence enhancers significantly reduce aqueous quenching and tune the wavelength of light emission.
