Electrophoresis > Electrophoretic Mobility Shift Assay (EMSA) Products & Reviews

The Odyssey® Infrared EMSA Kit coupled with IRDye® 700 EMSA oligonucleotides is an excellent alternative method to radioisotopic and chemiluminescent detection methods for EMSA analysis and visualization. Using IRDye EMSA reagents, assays can be completed in less than two hours with no gel transfer or film exposure.

A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol.

Amersham™ brand Typhoon™ laser-scanner platform provides exceptional data quality through extremely sensitive detection, high image resolution, and a very broad linear dynamic range. These versatile imaging systems support multiple imaging modes, including phosphor imaging, red/green/blue (RGB) and long and short wavelengths of near infrared fluorescence (NIR), as well as optical densitometry (OD) of proteins in stained gels.