The detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a s…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a s…
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectropho…
The CHEMICON Proteasome Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of proteasome activity in cell lysates or inhibitor screening. Chemicon's Proteasome Activity Assay Kit provides a simple and convenient means for assaying proteasome activity that recognize the substrate LLVY (Meng et al. 1999). The assay is based on detection of the fluorophore 7-Amino-4-methylcoumarin (AMC) after cle…
The CHEMICON uPA Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of uPA activity and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over a range of 0.05-50 units of uPA activity. The CHEMICON uPA Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA. Addition of this…
A Complete Solution for Functional Live Cell Assays Under Controlled Shear Flow—Great for Labs With an Existing Inverted Microscope
Innova Biosciences, based on the Babraham Research Campus, Cambridge UK, has launched a family of assay reagents called PiColorLock™, to facilitate the measurement of the activity of phosphatases, ATPases and other Pi-generating enzymes in microplates. Unlike the traditional "Malachite Green" method, which is prone to precipitation problems, PiColorLock reagent is specially formulated to give a stable end-point signal, meani…
AstraGene has been developed for the routine laboratory analysis of nucleic acids and proteins. With its specially developed through-the-tip measurement technology, your precious samples will never leave the pipette tip – speeding up analysis and protecting your sample. This simple system makes your work-flow as easy as just taking up the 2µl sample with the pipette, placing the pipette and tip in the holder and selecting th…
ColorKits are designed to facilitate the measurement of the activity of phosphatases, ATPases and other Pi-generating enzynes in microplate assays. Each Kit contains a phosphorylated substrate for a particular class of enzyme, together with a carefully optimised PiColorLock detection reagent, Pi standard, 2x96-well plates and a selection of buffer concentrates for optimisation of assay conditions with the enzyme of interest.…
PiColorLock Drug Discovery Kits are designed to facilitate the measurement of the activity of potential inhibitors of phosphatases, ATPases and other Pi-generating enzymes in microplates. The Kits combine a purified recombinant human enzyme target, phosphorylated substrate, Pi standard and 2 multiwell plates. Each Kit is also supplied with a carefully optimised PiColorLock detection reagent and a selection of buffer concentr…
Revolutionary 2DGE Software Analysis of single 2D gels - Dymension 1 is ideal for users looking for software to analyse spots between samples containing one 2D gel only. It offers a range of automatic image correction features and methods for resolving and analysing spots on single gels. The software can be easily upgraded to Dymension 2 or 3 if the proteomics needs of the laboratory change.
B-Pak, is a straightforward and rapid procedure for determining the concentration of protein in solution. Thanks to an optimized Bradford reagent formulation, B-Pak gives precise determination of protein concentration even in the presence of detergent, allowing the use of this kit for transfection experiments. In addition, this kit can be used for all protein concentration measurement due to a superior linearity of response: f…
An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…
Rapid Analysis of Multiple 2D Gel Sets DYMENSION 2 has all the features of DYMENSION 1 but has the added ability to detect and compare protein spot maps in multiple gel sets. The software offers many background correction and noise filtering features including a SYPRO® Ruby filter, which can detect and automatically remove spots caused by SYPRO® Ruby crystals adhering to the gel, thus saving users valuable time editing the…
Quick and Easy Analysis of all 2D Gel Applications The exciting DYMENSION 3 software has all the functionality of DYMENSION 2, so can be used to analyse multiple gel sets. In addition, DYMENSION 3 is capable of analysing multi-stained fluorescent gel images stained with up to three contrasting fluorescent labels, for example, Cy™2, Cy™3 and Cy™5. This feature allows users to analyse multiple sets of any type of 2D protein…
The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC enzymes. Unlike conventional assays that are dependent upon a s…
F2H®-Kit Basic for effortless imaging of interactions between GFP- and RFP-tagged proteins of interest and domains thereof in live mammalian cells. The Fluorescent Two- Hybrid (F2H®) Technology is a fast and simple way to study protein-protein interactions (PPIs) by conventional fluorescence microscopy. Its direct optical readout is optimal for high-content analysis (HCA) and screening for PPI inhibitors.F2H® Principle: F…
ProteinCenter™ is a web-based data interpretation tool that enables scientists to compare and interpret data sets in minutes instead of months ProteinCenter is a web-based tool that enables scientists to compare and interpret proteomics data sets in minutes and overcomes the hurdle of proteins existing under different names and accession keys in different databases. Regularly updated from all major protein databases, ProteinC…
Protein Analysis Software - the Next GenerationCLC Protein Workbench creates a software environment enabling users to make a large number of advanced protein sequence analyses, combined with smooth data management, and excellent graphical viewing and output options. Protein Workbench sets new standards for bioinformatics software with its overall graphical display, usability driven and user-friendly interface, while featuring…
Free Bioinformatics Software Not a demo. Not scaled down. No spyware. No need to register. Yet still a wide variety of features available - and - free! CLC Free Workbench creates a software environment enabling users to make a large number of bioinformatics analyses, combined with smooth data management, and excellent graphical viewing and output options. Some of the many bioinformatics analyses: • Interactive restriction site…
Our industry leading bioanalytical solutions have helped our clients develop new peptide therapeutics to improve the quality of life. Combining our experience and cutting-edge technology, we have has developed and validated methods using LC-MS/MS to quantify levels of peptides in plasma at levels as low as 10 pg/mL.
Designed to identify the physical interactions of a miRNA with endogenous mRNA transcripts
Simple, sensitive method for detecting changes in histone methylation levels from purified core histones or histones isolated by acid extraction
xMAP® Technology uses color-coded microspheres to detect multiple protein or oligonucleotide analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Depending on your targets and the application, there are five different microsphere types to select from to produce the best experimental results. MagPlex-TAG™ are oligonucleotide-coupled ma…
xMAP® Technology uses color-coded microspheres to detect multiple protein or oligonucleotide analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Depending on your targets and the application, there are five different microsphere types to select from to produce the best experimental results. MicroPlex® are carboxylated polystyrene mi…
