BioFlux 200
A Complete Solution for Functional Live Cell Assays Under Controlled Shear Flow—Great for Labs With an Existing Inverted Microscope
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BioFlux provides a fully integrated solution for running biologically relevant cellular assays under shear flow. The system utilizes Fluxion's Well Plate Microfluidics™ (WPM) to provide high content results from a convenient well-plate format. Precise control over shear flow enables a wide variety of cellular and microbiology applications which call for vascular emulation, mechanical stress, compound/buffer exchange and high content analysis.
BioFlux enables a wide variety of cell-based assays in the fields of immunology, vascular biology, microbiology, cancer research, stem cells, and more. Many of these applications benefit from the ability to create a physiologically-relevant shear flow profile, such as platelet adhesion, cell adhesion, rolling velocity, transmigration, and stem cell differentiation.
Other applications benefit from the ability to introduce compounds and gradients in a time-resolved manner, such as wound healing, chemotaxis, pharmacology and neurite-outgrowth assays. The BioFlux 200 System comes with all of the necessary hardware and software to start running shear flow assays with an existing inverted microscope. Comes with controller, pressure interface, software, and accessories. Works with BioFlux Plates, which are microfluidic well plate devices for flow assays.
Leukocyte Transmigration
Transmigration of leukocytes from the lumen of the blood vessels to the underlying tissue is a key process in immunosurveillance and in the response to inflammation and injury in the normal function of the immune system. However, in inflammatory diseases and cancer, transmigration of cells to underlying tissues is a gateway to metastasis and morbidity. The significance of transmigration of unwanted cells to underlying tissues and through the blood brain barrier from the vasculature in disease states makes it an important target for drug development. As such, transmigration is a prime target for inflammation and oncology drug discovery. Download this application note to read about the use of BioFlux System to study live cell transmigration.
High Throughput Cell Adhesion: Screening of VLA-4 Antagonists Under Physiological Shear Flow Conditions
Cell adhesion governs countless biological phenomena in health and disease. This application note presents a screen of three monoclonal antibodies directed against very late antigen-4 (VLA-4) for assessment of adhesion reduction to vascular cell adhesion molecule -1 (VCAM-1) in the Bioflux™ System. This screen analyzed the number of cells adhering to the VCAM-1 substrate, as well as the morphological characteristics of attached cells. The time to perform the entire experiment from coating the plate with VCAM-1 to manual data collection is reduced to 3 hours in comparison to 6-18 hours for a standard microplate adhesion inhibition assay.
Microfluidics technology provides physiologically relevant cell-based models for high-throughput drug discovery
Jeff Jensen, CEO of Fluxion Biosciences, describes how microfluidics technology fuels advances in drug discovery through physiologically relevant cell-based assays
