Western blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The ChIPAb+ Trimethyl-Histone H3 (Lys4) set includes the anti-trimethyl-histone H3 (Lys4) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 166 base pair region within the promoter of the human GAPDH gene. The trimethyl-histone H3 (Lys4) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of tr…
The ChIPAb+ Acetyl-Histone H3 set contains purified rabbit polyclonal antibody and the normal rabbit IgG antibody, which can be used to demonstrate that the acetyl-histone H3 antibody is capable of precipitating acetyl-histone H3 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.
The ChIPAb+ RNA Pol II set includes the RNA Pol II antibody, the negative control antibody (mouse IgG), and qPCR primers flanking the human GAPDH promoter, yielding a 166 bp product. The RNA Pol II and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of RNA Pol II associated chromatin.
Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Trimethyl-Histone H3 (Lys27) set includes the Anti-Trimethyl-Histone H3 (Lys27) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 170 bp region within the promoter of the murine p16 promoter. The Trimethyl-Histone H3 (Lys27) and negative control antibodies are supplied in a scal…
The ChIPAb+ Acetyl-Histone H4 set includes purified rabbit polyclonal antiserum and the normal rabbit IgG antiserum, which can be used to demonstrate that the acetyl-histone H4 antibody is capable of precipitating acetyl-histone H4 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.
The ChIPAb+ Dimethyl-Histone H3 (Lys9) set includes the anti-dimethyl-histone H3 (Lys9) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 110 bp region within the promoter of the human β-globin gene. The dimethyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of dimethyl-…
The ChIPAb+ Ubiquityl-Histone H2B, (clone 56) set includes the Ubiquityl-Histone H2B, (clone 56) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 213 bp region within the coding region of the human GAPDH gene.The ubiquityl-Histone H2B and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Ubiqu…
The ChIPAb+ Acetyl-Histone H3 (Lys9) set includes the anti-acetyl-histone H3 (Lys9) antibody, a negative control antibody (purified Rabbit IgG), and qPCR primers flanking an Sp1 binding site in the human p21 (WAF1/CIP1/CDKN1A) promoter, amplifying a 105 base pair PCR product. The acetyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate…
The ChIPAb+ Dimethyl-Histone H3 (Lys4) set includes the Anti-dimethyl-Histone H3 (Lys4) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 213 bp region within the coding region of the human GAPDH gene. The dimethyl-histone H3 (Lys4) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of dimethyl…
The OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
OxyBlot™ Protein Standard is composed of five proteins (phosphorylase B, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor) containing 1-3 dinitrophenylhydrazone (DNP) residues per protein. These attached DNP moieties permit the visualization of the proteins by the detection system of the OxyBlot™ Protein Oxidation Detection Kit. OxyBlot™ Protein Standard serves as an internal control for the electrophoresis, western trans…
TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum of 653 nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing…
Bløk Noise Cancelling Reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimized to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection. Bløk buffers are ideal for use with Immobilon®-P Western blotting membranes and the SNAP i.d.® system, and are also compatible with standard Western blotting techniques.
The Luminata ELISA HRP chemiluminescent substrates provide three major advantages:High sensitivity for detecting as little as 0.1 pg of proteinBroad dynamic detection range (up to 2,500-fold), enabling a wide range of quantitation.Flexibility to adjust ELISA signal strength
The Visualizer™ Western Blot Detection Kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.
The Visualizer™ Western Blot Detection Kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.
Gel blottingSpecifically designed to work with the 120 mini-vertical gel system simple and fast gel blotting with low buffer requirements and fast transfer rates. Product detail:C140 Mini Blot ModuleSpecifically designed to work with the 120 Mini-Vertical Gel System. The Mini Blot Module is a small tank blotter that incorporates the best features of a semi-dry blotter (low buffer requirements and fast transfer rates), with non…
AMRESCO now offers a way for you to remove Ethidium Bromide, Coomassie Blue, and other biological dyes and stains from solution for easy, safe disposal. AMRESCO Destaining Bags effectively extract milligram quantities of ethidium bromide from solution without exposing research personnel to corrosive agents or contaminated substrates. The special adsorbent mixture retains dye molecules in a convenient bag for removal and inci…
Novel protein markers for visible tracking & accurate sizing on any Western blotTrail Mix™ Western Markers consist of the Perfect Protein™ Western Markers supplemented with a group of three prestained indicator proteins to allow direct visualization of protein mobility during electrophoresis. Trail Mix Western Markers and Western Blot Kits have all of the features of Perfect Protein Western Markers and Western Blot Kits, plus…
Chromobodies® - Your perfect reagents for cellular research including High-Content Analysis. Chromobodies are fluorescent nanoprobes suitable for real time analyses of endogenous cellular structures and processes in live cells. Chromobodies® are a novel species of extremely small intracellular antibodies. They are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a f…
Unique design that includes an intelligent built-in power supply which automatically monitors the transfer status, stopping the transfer before the stack overheats.The Semi-Dry Transfer Units support as many as two layers of gels being transferred simultaneously. The TE70XP has a 14 x 16 cm maximum transfer area which can handle up to four mini gels using the stacked format. The TE77XP has a 21 x 26 cm transfer area for a capa…
Quickly and efficiently transfers proteins and nucleic acids from small gels to nylon, nitrocellulose or PVDF membranes.The TE62 is designed to accept up to four cassettes simultaneously with cooling. Each cassette holds a 15 x 21cm gel, or as many as four 7 x 10 cm mini gels. TE62 Mighty Small Transfer Tank with Cooling Chamber Features: Uniform and strong electric field–supports efficient and even transfers Color code…
Recently redesigned to accommodate up to 4 electroblotting cassettes, the Scie-Plas TV100-EBK and TV400-EBK electroblotters provide a cost-effective and safe and easy to use system for the transfer of proteins and nucleic acids from 10 x 10cm mini and 20.5 x 20cm maxi gels. Both units are supplied with asymmetric colour-coded cassettes as a unique design feature to prevent them from being inserted into the tank in the wrong o…
Quickly grab quantitative images of your blots or gels right on your bench with the Omega Lum C. With its broad dynamic range and sensitive detection, you can’t get better data from your experiments anywhere else. And it is so easy – you’ll never want to use film again. The Omega Lum c is optimized for superior detection of chemiluminescent Western blots. A wide dynamic range and fast imaging speeds mean that you will spend l…
With the Omega Lum™ G snap an image of your gel or blot, get your data and go! Our SmartCapture technology simplifies imaging, so all you need is to pick your application, exposure, and capture your image. You will have a publication-ready image in seconds, and can get back to what’s important – your work. The Omega Lum G revolutionizes gel imaging by combining fluorescent gel and chemiluminescent imaging into one compact sys…
