Real-Time PCR (qPCR) Mastermixes and Kits Products & Reviews

qPCRBIO Probe Blue Mix is a universal probe kit that includes a non-reactive blue dye for easy sample visualisation and enhanced pipetting accuracy. Whether your application is for a singleplex or multiplex expression study or for genotyping and allelic discrimination analyses, qPCRBIO Probe Blue Mix is the robust choice for all your probe-based real-time PCR needs.

qPCRBIO Probe 1-Step Virus Detect is designed for ultra-sensitive detection of viral RNA by 1-step RT-qPCR using sequence-specific probes.

Lyo-Ready Probe 1-Step Kit is a lyophilisable (freeze-dryable) RT-qPCR kit designed for ultra-sensitive detection of RNA sequences.

Air-Dryable Probe 1-Step Mix is a versatile all-in-one, air-dryable 4x RT-qPCR reagent mix for ultra-sensitive detection of RNA or DNA sequences.

RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara® Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

Clara® HRM Mix delivers maximum specificity to your high resolution melt (HRM) curve analyses. Accurately detect genetic mutations, quickly identify genotypes based on SNPs, or calculate percent methylation of a target region with HRM analysis.

DNA-free Taq DNA polymerases for contamination-free analysis

Universal 16S rDNA real-time PCR assay for sensitive detection of bacterial DNA

Universal 18S rDNA real-time PCR assay for sensitive detection of fungal DNA

This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products. Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for interca…

KAPA hgDNA Quantification and QC Kits contain all the reagents needed for the qPCR-based quantification and quality assessment of human genomic DNA samples prior to NGS library construction. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR, as well as a pre-diluted set of DNA standards and primer premixes targeting different portions of a highly conserved single-copy human…

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis.  KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent rea…

High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in nucleic acid sequences. The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system, and EvaGreen® saturating dye optimized for maximum discrimination between sequence variants.   Features: Maximize differences in sequence variants Engineered to maximize differe…

10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 1,000 x 20 µl reactions

500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO

2,000 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2

200 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq DNA polymerase

25 x 20 µl reactions, 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

100 x 20 µl reactions, 4 x 100 µl 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

100 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 6 mM MgCl2, 50 U/ml iTaq DNA polymerase

500 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 6 mM MgCl2, 50 U/ml iTaq DNA polymerase

1,000 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq DNA polymerase, 6 mM MgCl2

200 µl, premixed dNTP solution, 10 mM each (dATP, dCTP, dGTP, dTTP)

5,000 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 25 ml each 10x PCR buffer and 50 mM MgCl2

2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions