PCR and Thermal Cycling Products & Reviews

Primer-only premixed assays for analyzing gene expression using intercalating dyes

RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara® Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

Clara® HRM Mix delivers maximum specificity to your high resolution melt (HRM) curve analyses. Accurately detect genetic mutations, quickly identify genotypes based on SNPs, or calculate percent methylation of a target region with HRM analysis.

For nanoplate-based digital PCR applications

Accumax range of PCR plates & seals are the outcome of our impeccable standards of quality established throughout the manufacturing process resulting in the plates & seals that gaurantee precise and error free results every time.

QIAseq FastSelect: Increase your unique reads, discover more biology

QIAseq FastSelect: Increase your unique reads, discover more biology

The Applied Biosystems TaqPath Respiratory Viral Select Panel is a multiplex, real-time PCR (qPCR) in vitro diagnostic (IVD) test for identifying five common respiratory viruses in under three hours. The CE-IVD panel includes targets for adenovirus, human metapneumovirus, rhinovirus/enterovirus,* and parainfluenza** virus. 

The  therascreen  KRAS RGQ PCR Kit is a real-time, qualitative in vitro diagnostic test for the detection of 7 somatic mutations in the  KRAS  oncogene using a sample of DNA extracted from formalin-fixed, paraffin-embedded (FPPE) non-small cell lung cancer (NSCLC) tissue and colorectal cancer (CRC) tissue.

A comprehensive kit for efficient multiplex RT-qPCR in a single reaction tube. Ideal for multiplex (4-5) assays using dual-labelled hydrolysis probes, works with standard and fast cycling.

A comprehensive kit for performing dye-based RT-qPCR swiftly and reliably in a single tube. No carryover contamination and compatible with standard and fast cycling.

Reliable multiplex qPCR kit. Optimised for amplifying low copy DNA targets. Ideal for multiplexed reactions of 4 or 5 targets without compromising performance.

Ideal for quantification of any DNA template (gDNA, cDNA, viral DNA), amplifies low copy number samples with ease. Utilises our intercalating fluorescent dye, MicroGREEN, that binds to double stranded DNA, making it the perfect choice for qPCR & melt curve analysis.

The InviScreen ® Norovirus Detection Kit provides a real-time RT-PCR method for the qualitative detection of norovirus genogroups I (GI) and II (GII) in food samples. The method is based on ISO 15216 and detects specific regions in the conserved 5′ end of the ORF1-ORF2 junction. The method is applicable to test samples of foodstuffs and surface swabs. The kit includes Infectious Bronchitis Virus (IBV) as a process control vi…

The InviScreen ® STEC Detection Kit provides a real-time PCR method for the qualitative detection of STEC E. coli in food samples. The method is based on ISO 13136 and detects the presence of the stx1 and stx2 genes (associated with cytotoxicity) and the eae gene which is associated with the capability of the bacteria to adhere to the intestinal epithelium and cause disease. The method is applicable to products intended for h…

The InviScreen ® Clostridium Botulinum Detection Kit is specifically designed for the qualitative detection of the specific botulinum neurotoxins (BoNTs) A, B, E and F of Clostridium botulinum in food, feed, and environmental samples. The real-time PCR procedure for detecting the presence of genes encoding BoNT type A (BoNT/A), type B (BoNT/B), type E (BoNT/E) and type F (BoNT/F) complies with the ISO/TS 17919 standard. The…

The InviScreen ® GMO Detection is a molecular biology tool that allows to easily detect genetically modified organisms (GMOs) using a fast, ISO 21569 standard-based, real-time PCR detection method based on the detection of the pCaMV35S, tNOS and pFMV genes. The kits can be used with a variety of starting materials, and benefits from the InviSorb® extraction kits that offer efficient protocols for DNA purification.

The InviScreen ® Allergen Detection is a premium quality molecular biology tool to provide the general laboratory and research personnel an easy and ready-to-use kit for the detection of specific allergen species in food and feed samples. For DNA purification prior to testing, the Invisorb ® extraction kits offer efficient protocols for various starting materials. Benefit from our real-time PCR detection technology and get ac…

Swift, cost-effective qPCR detection. Compatible with a wide range of qPCR instruments and mastermixes. Heightened sensitivity and outstanding reproducibility due to high fluorescence. A robust, thermally stable dye endures cycling and freeze-thaw cycles exceptionally well. 

VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

The Mastercycler X40 combines features that raise your standard in endpoint PCR to a new level. In addition to classic features such as a twelve-slit gradient and outstanding block temperature homogeneity, there is a simple, intuitive user interface, a touchscreen and a SafeLid lid that protects your samples from evaporation.

The MegaMix Gold One-step RT-PCR kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix Gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.

96-well, thermal cycler for PCR with cables; for research use only.

Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cu…

Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Verichec…