High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
LiChrospher® PAH is a highly efficient and selective HPLC column especially designed for the high resolution separation of 16 PAH (polycyclic aromatic hydrocarbons) according to EPA 610 and 550 + benzo(e)pyrene + perylene. LiChrospher® PAH is based on a special modified RP-18 silica gel to achieve highly resolved PAH separations. LiChrospher® PAH can be used for both, the isocratic separation of 6 PAH according to the German…
For reproducible reversed phase separation: LiChrospher® RP-phases are reliable and versatile traditionally produced spherical silica carrier with reversed phase properties. They are well suited for the chromatography of acidic, neutral and weakly basic compounds, substances found frequently in all analytical fields. A selectivity and performance guarantee makes sure that these parameters remain constant form batch to batch a…
Excellent separations even with basic compounds: LiChrospher® RP-select B is a versatile reversed phase sorbent based on spherical silica particles with excellent properties for the determination of basic substances, but with still good properties for the determination neutral and acidic substances. LiChrospher® RP-select B is optimised in order to prevent any secondary interactions with basic substances and guarantees that…
High-resolution separations of peptides and tRNA molecules: LiChrospher® WP 300 RP-18 is a highly selective and reliable HPLC column for the separation of peptides and low molecular weight proteins. LiChrospher® WP 300 RP-18 enables the tailing-free separation of basic compounds and is excellently suited for the separation of tRNA molecules. High recovery rates are achieved, especially in the case of highly hydrophobic pepti…
The GPC Quattro is designed for the clean-up of complex animal tissues, food products and environmental samples by separating high molecular weight matrixes such as lipids, carbohydrates, pigments, etc., from analytical samples. Beneficial features of the GPC Quattro include: • Fourfold increase in productivity in comparison to traditional single-column instruments• High throughput • Economical price • Full error management…
UHPLC quaternary solvent delivery system with operating pressure to 1250 barThe Thermo Scientific Accela high-speed chromatographic system provides fast, efficient HPLC and U-HPLC in One Efficient, Limitless System. The Accela™ 1250 High Speed LC pump provides accurate and precise flow and gradients under all operating conditions with minimum pulsation. Features of the UHPLC Accela 1250 pump: • Quaternary capabilities with f…
The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations: • Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons • Non-circular drive gears provide smooth, uniform eluent delivery • User-programmable flow rate calibration and compressibility compensation factors ensure straightforward pump operation qualification • Tests t…
Allows simpler, time saving methods for reversed phase chromatography of basic compounds: Purospher® RP-18 is a versatile HPLC column designed for the separation of problematic basic compounds with simple neutral eluents. This results in less time and cost because of simpler method optimization. Purospher® RP-18 is based upon a high purity, metal free silica. The multi-step chemical modification and deactivation of the surface…
Excellent peak symmetry with either basic or strongly acidic compounds: Purospher® RP-18 endcapped is a versatile HPLC column producing excellent peak shapes, designed for both the separation of basic compounds with simple neutral eluents and for the elution of strongly acidic compounds. Excellent separations in a shorter period of time with very good peak symmetry saves time and money. The excellent balance of Purospher® RP-1…
Purospher® STAR NH2 is a high purity aminopropyl modified stationary phase.
Purospher® STAR RP-18 endcapped is a versatile high purity reversed phase column increasing the productivity in HPLC. Purospher® STAR RP-18 endcapped provide the power to separate the most difficult of compound mixtures. With up to 150.000 N/m for 3 µm and 100.000 N/m for 5 µm particle size, Purospher® STAR RP-18 endcapped will ensure good base line separations.
Purospher® STAR RP-8 endcapped is a versatile high purity reversed phase column increasing the productivity in HPLC.Purospher® STAR RP-8 endcapped provide the power to separate the most difficult of compound mixtures. With up to 150.000 N/m for 3 µm and 100.000 N/m for 5 µm particle size, Purospher® STAR RP-8 endcapped will ensure good base line separations.
A “Brush-type” phase with broad applicability for separation of enantiomers: Whelk-O 1 is a reliable chiral HPLC column of high solvent stability and broad applicability. Whelk-O 1 is a “brush-type” phase according to the concept of Pirkle with (3R, 4R)-4-(3,5-dinitrobenzamido)-1,2,3,4-tetrahydrophen-anthren or (3S, 4S)-4-(3,5-dinitrobenzamido)-1,2,3,4-tetrahydrophen-anthren as chiral selector with high enantoselectivity for a…
The Biochrom 31+ Amino Acid Analyzer is used by reference labs, food and feedstuff manufacturers, pharmaceutical and industrial labs worldwide. It can rapidly deal with complex samples, requiring virtually no sample preparation. The system accurately detects and quantifies amino acids and their derivatives in protein hydrolysates. Applications include: proteomics, drug synthesis, infusion fluids, and quality control off food…
The Biochrom 32+ Oxidized Hydrolysate System is designed for the analysis of complex protein hydrolysates such as those found in food and feedstuffs. This system meets the requirements of the standard methods from AOAC and the EU Commission Directive 98/64/EC. It is trusted by food, beverage and feedstuffs analysis laboratories worldwide. It accurately detects and quantifies amino acids and their derivatives in complex sam…
