Genome Analysis Products & Reviews

Thermo Fisher Scientific offers all ABgene PCR Master Mixes in a convenient pre-aliquoted format. This time-saving solution allows for more reproducible results by minimizing reagent handling steps and pipetting errors. Our Pre-aliquoted Master Mixes can be tailored to your very needs as custom primers can be added on request, and you can choose a plate or tube format that will suit your experimental set-up. Pre-aliquoted PCR…

CLC Combined Workbench includes all features and functions of CLC Free Workbench, CLC Protein Workbench, and CLC Gene Workbench. The program sets new standards for bioinformatics software with its overall graphical display and usability driven, user-friendly interface. In addition, it features a number of unique and innovative bioinformatics tools for supporting advanced biochemistry and molecular biology lab research. CLC Com…

CLC bio presents its new software module for Multilocus Sequence Typing (MLST). MLST is a portable and precise state-of-the-art technique for typing bacteria and yeast isolates. It is based on DNA sequence data from a number of house-keeping genes. For each gene, the experimental data is compared to a database of known alleles and a fi nal sequence type is assigned by combining information from all genes. Easy The CLC MLST Mod…

The CHU25 Maxi-Plus cooled horizontal gel unit is designed specifically for rapid high throughput screening of nucleic acids, particularly PCR products and in genotyping studies.In addition to having the same 25 x 30cm gel-casting tray, 624-sample throughput, buffer recirculation ports and gel-casting features of its HU25 counterpart, the CHU25 has a labyrinthine cooling base situated directly within the tank. Once the gel is…

With the all new CLC RNA Workbench, CLC bio brings you complex analysis of RNA molecules in a fully integrated, user-friendly and graphically advanced bioinformatics framework. Advanced The CLC RNA Workbench gives the user easy access to a range of complex algorithms and options. RNA secondary structures can be predicted using state-of-the-art free energy minimization algorithms and parameters, thermodynamic and statistical d…

IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon.

X-GAL (5-Bromo-4-Chloro-3 Indolyl-Beta-D-thiogalactopyranoside) is a chromogenic substrate for Beta-galactosidase that turns blue upon hydrolysis and is regularly used for the blue/white color selection assay. Bacteria with a lac+ phenotype grow as blue colonies on plates containing X-GAL.

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression.

Axygen DNA Markers for Gel Electrophoresis - Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These DNA Markers for Gel Electrophoresis are formulated to run accurately and to provide crips band patterns. The two Axygen DNA Markers for Gel Electrophoresis require no additional preparation and contain b…

The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…

Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.…

Thermo Scientific Verso 1-step RT-qPCR Kits for probe chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment…

Thermo Scientific Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I trea…

Thermo-Start Taq DNA Polymerase is a hot-start enzyme combined with an optimized buffer system that gives superior specificity and sensitivity. The chemically modified Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction. This prevents nonspecific amplification during reaction setup, eliminating smearing and the formation of nontarget bands.…

Thermo Scientific Verso 1-Step RT-PCR system is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to c…

Thermo Scientific Gene Frame products feature a dual strength adhesive system which facilitates the easy removal of the frames after use. The adhesives on either side of the frame are of differing strengths; the side which adheres to the glass has an easy release adhesive whereas the reverse, which sticks to the coverslip, forms a stronger bond. This means that when the coverslip is removed, due to the disparity in the strengt…

Thermo Scientific Superladders are derived from cloned and purified DNA fragment repeats. A range of sizes are available. Superladder-Low 20bp LadderSize range: 20-1000bpNumber of bands: 50 Superladder-Low 100bp Ladder Size range: 100-1000bp Number of bands: 10Superladder-Mid 100bp Ladder Size range: 100-3000bp Number of bands: 30Superladder-Mid 200bp Ladder Size range: 200-6000bp Number of bands: 30

Thermo Scientific has a wide range of DNA digest markers for low and high molecular weight products. Markers are supplied with loading dye allowing the same loading dye to be used for the sample and the marker. Markers available:PhiX174 RF DNA Hae III MarkerPhiX174 RF DNA Hinc II MarkerPhiX174 RF DNA Hinf I MarkerpBR322 hae III MarkerpBR322 Msp I MarkerLambda Hind III MarkerLambda EcoRI/ Hind III Marker

The Thermo Scientific Dye Terminator Removal Kit is designed to perform the essential removal of unbound fluorescently-labelled dideoxy-ribonucleotides (ddNTPs) and excess salt from sequencing reactions prior to sequence analysis. The kit includes a 96-well plate containing pre-hydrated gel separation matrix consisting of spheres with uniformly sized pores. Sequencing reactions are passed through the separation matrix, which r…

The Thermo Scientific Separation Plate can be used for gel filtration applications (eg. dye terminator removal) when a suitable matrix is added. Features • 96 wells containing polyethylene frits, pore size of 30–45µm • 0.6ml well volume • Ready-to-use microplate format • Suitable for centrifugation

Thermo Scientific Tth DNA Ligase catalyses the ligation between double-stranded DNA molecules with 3’ hydroxyl and 5’ phosphate termini. The enzyme will also repair single-stranded nicks in double-stranded nucleic acids. Features • Ligates double-stranded DNA • Repairs single strand nicks in double-stranded DNA

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I. Highlights • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers A…

Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity. Highlights • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1) • Active in Therm…

Thermo Scientific T4 Polynucleotide Kinase (PNK) is a tetrameric protein composed of four identical subunits. It is widely used in molecular biology to radiolabel nucleic acids and to add a phosphate to the 5'-end of dephosphorylated nucleic acids to facilitate ligations. Features • Phosphorylates 5’ ends • Transfers 32P to DNA and RNA