PCR and Thermal Cycling Products & Reviews

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Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Axygen® 0.5 mL Thin Wall PCR Tubes with Flat Cap, Clear, Nonsterile, 1000/Cs

Corning Life Sciences

Axygen® 0.5 mL polypropylene thin wall PCR tubes with flat caps are designed to fit standard 96 well blocks and are available in an assortment of colors. Ultra-thin and consistent wall thickness allows precise thermal transfer and even insertion cap ensures a tight fit to prevent evaporation. RNase-/DNase-free Nonpyrogenic Nonsterile Manufactured with 99.9% pure virgin polypropylene

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Axygen® 0.5 mL Maxymum Recovery® Thin Wall PCR Tubes with Flat Cap, Clear, Nonsterile

Corning Life Sciences

Axygen® 0.5 mL polypropylene thin wall PCR tubes with flat caps are designed to fit standard 96 well blocks and are available in an assortment of colors. Ultra-thin and consistent wall thickness allows precise thermal transfer and even insertion cap ensures a tight fit to prevent evaporation.RNase-/DNase-freeNonpyrogenicNonsterileManufactured with 99.9% pure virgin polypropyleneMaxymum Recovery technology for ultra-low retenti…

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Axygen® PCR 35 µm Aluminum Sealing Film For Thermal Sealing and General Sealing Purposes, Nonsterile

Corning Life Sciences

Axygen® microplate sealing films are designed for applications ranging from PCR and Real-Time PCR to ELISA and cell culture. Axygen’s sealing films are the easiest and most cost-effective method for sealing plates. They can be used in wide temperature ranges, making them suitable for many applications.Provides exceptional seal on multiple well plates with a wide range of reagents and temperaturesWhen used in conjunction with A…

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Axygen® 80 µm AxySeal Sealing Film for Tissue Culture, Short-Term Storage and Transportation, Nonsterile

Corning Life Sciences

Axygen® microplate sealing films are designed for applications ranging from PCR and Real-Time PCR to ELISA and cell culture. Axygen’s sealing films are the easiest and most cost-effective method for sealing plates. They can be used in wide temperature ranges, making them suitable for many applications.Polyester based with uniformly and consistently applied acrylic adhesive to eliminate the edge effect in sensitive ELISA assays…

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Axygen® Sealing Film Roller

Corning Life Sciences

Axygen® sealing film roller is a durable and efficient roller ideal for applying sealing films to any plate. Sturdy plastic handle and semi-hard roller head are ideal for complete and easy applications of film.Ideal for the application of all PlateMax™ sealing films

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Axygen® 80 µm AxySeal Sealing Film for Tissue Culture, Short-Term Storage and Transportation, Sterile

Corning Life Sciences

Axygen® microplate sealing films are designed for applications ranging from PCR and Real-Time PCR to ELISA and cell culture. Axygen’s sealing films are the easiest and most cost-effective method for sealing plates. They can be used in wide temperature ranges, making them suitable for many applications.Polyester based with uniformly and consistently applied acrylic adhesive to eliminate the edge effect in sensitive ELISA assays…

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Axygen® 60 µm CyclerSeal Sealing Film for Storage and PCR Application, Nonsterile

Corning Life Sciences

Axygen® microplate sealing films are designed for applications ranging from PCR and Real-Time PCR to ELISA and cell culture. Axygen’s sealing films are the easiest and most cost-effective method for sealing plates. They can be used in wide temperature ranges, making them suitable for many applications.Exceptional seal across entire plate safeguards samples during transport or storageWhen used with Axygen's compression mat, thi…

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Axygen® MaxyGene™ II Thermal Cycler with 96 well block, 110V

Corning Life Sciences

The new MaxyGene II Thermal Cycler replaces our successful and reliable MaxyGene. It brings increased speed and advanced features, providing the premium performance you have come to expect from Axygen brand products.Unique flexible programmingRapid run timesImproved workflow over traditional gradient cyclersRamping rates up to 5°C/sec.Simple user interfaceAdjustable heated lid accommodates strips, tubes and microplatesPre-prog…

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KAPA hgDNA Quantification and QC Kit

Kapa Biosystems, Inc.

KAPA hgDNA Quantification and QC Kits contain all the reagents needed for the qPCR-based quantification and quality assessment of human genomic DNA samples prior to NGS library construction. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR, as well as a pre-diluted set of DNA standards and primer premixes targeting different portions of a highly conserved single-copy human…

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KAPA SYBR® FAST qPCR Kits

Kapa Biosystems, Inc.

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis.  KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent rea…

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KAPA SYBR FAST One-Step qRT-PCR Kits

Kapa Biosystems, Inc.

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence.  The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol. KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV r…

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KAPA HRM FAST

Kapa Biosystems, Inc.

High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in nucleic acid sequences. The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system, and EvaGreen® saturating dye optimized for maximum discrimination between sequence variants.   Features: Maximize differences in sequence variants Engineered to maximize differe…

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KAPA HiFi Uracil+

Kapa Biosystems, Inc.

KAPA HiFi DNA Polymerase provides high fidelity next-generation sequencing library amplification with the highest yield, lowest bias, and most uniform sequence coverage available. These characteristics are retained in KAPA HiFi Uracil+ DNA Polymerase, a modified version of KAPA HiFi engineered to tolerate uracil residues. The new enzyme is particularly well suited for applications employing bisulfite DNA conversion, which t…

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KAPA2G Fast Multiplex

Kapa Biosystems, Inc.

KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzyme derived through a process of directed evolution. KAPA2G FAST HotStart DNA Polymerase is an antibody-mediated hot start formulation engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes,…

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KAPA3G Plant PCR Kits

Kapa Biosystems, Inc.

KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolu…

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KAPA2G Fast PCR Kits

Kapa Biosystems, Inc.

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.   Features: Reduce cycling times up to 75% Extension times as low as 1 sec per kilobase Broad c…

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KAPA Mouse Genotyping Kits

Kapa Biosystems, Inc.

From tail to type in 1 hour! KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast…

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KAPA Long Range PCR Kits

Kapa Biosystems, Inc.

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the fi…

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KAPA Blood PCR Kits

Kapa Biosystems, Inc.

KAPA Blood DNA Polymerase is a second-generation enzyme derived through a process of directed evolution, and is the first DNA polymerase engineered specifically for the amplification of DNA directly from whole blood. DNA fragments may be amplified directly from reactions containing 1%-20% (v/v) whole human blood without pretreatment of blood samples or DNA isolation, significantly reducing contamination risk, turnaround tim…

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KAPA Taq PCR Kits

Kapa Biosystems, Inc.

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq i…

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KAPA dNTPs

Kapa Biosystems, Inc.

High-quality, molecular-grade dNTPs. Kapa offers premium grade dNTPs for PCR. KAPA dNTP Mix contains dATP, dCTP, dGTP, and dTTP supplied at a concentration of 10 mM each.   Applications: Standard PCR qPCR cDNA synthesis DNA sequencing DNA labeling Microarrays

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