High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Our new certified vial, cap and septa products eliminate "fit" and septa contamination concerns associated with other brands.By taking these extra manufacturing and testing steps, we can ensure:• Caps thread properly onto vials, providing an air-tight seal• Syringe needles are not damaged by irregular vial bottom thickness• Minimal contamination from septa impurities.Quality is built into each component of the Supelco certifie…
Diamond Analytics High Performance Liquid Chromatography (HPLC) columns expand the existing range of analytical capabilities in separation science by providing diamond-based solutions that allow for the exploration of novel chemistries. Flare columns have unique selectivity and are pH stable. They can run at elevated temperatures. They also provide increased longevity, without compromising efficiency. Flare columns can be reg…
Cogent Bidentate C18™ HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a straight chain C18 hydrocarbon bonded directly to the Silica Hydride surface with two separate points of attachment. The manufacturing of the Silica makes these columns virtually indestructible.
Flexible Multiport System for DURAN® GLS 80 Wide Mouth Bottles. The multi-port system consists of a GLS 80® screw cap with four ports. The tubes are fed through these ports and secured with inserts and screw connections. There is a choice of 5 inserts, each with different internal diameter depending on the application. Unused ports can be fitted with a blanking cap, if necessary. Sterile pressure compensation can be achieved…
GL 45 Screw caps with two or three ports. The connection system for DURAN® laboratory bottles are screw caps with GL 45 threads and two or three ports. Liquids can be added to or removed from bottles using the tube connections. Using a universal GL 14 tube adapter and different inserts, four different tube diameters can be used.This flexible, modular system provides the option of combining various components according to need…
DG safety caps are high-grade, flexible connection systems which have been designed specially for handling aggressive, gas-forming liquids. Available with 1, 2 or 3 connection ports with optional stopcocks, DG safety caps are particularly suitable for pharmaceutical and chemical applications. The safety caps come complete with v-ring seal (inside diameter 3.2 mm) and are ready for immediate use. V-ring seals are optionally ava…
The pentafluorophenylpropyl (PFP) stationary phase of Ascentis® Express F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, some…
Wheaton peristaltic pumps are used for dispensing and general liquid handling purposes. The pumps are delivered ready to use with tubing kits included. The pumps can be programmed to dispense measured aliquots into culture plates, vials, tubes or bottles. The Omnispense units have additional features including speed ramping and totalizer/flow mode. Features: Includes complete pump, instruction manual and tubing kits. Works…
Wheaton peristaltic pumps are used for dispensing and general liquid handling purposes. The pumps are delivered ready to use with tubing kits included. The pumps can be programmed to dispense measured aliquots into culture plates, vials, tubes or bottles. Includes complete pump, instruction manual and tubing kits. Works with 2.3mm thick tubing. Additional pump heads and foot switches available. Features: Icon driven user in…
Tri-Fitt Ultra-High Pressure Fittings for HPLC applications that require no leaks at pressures at or above 20,000 psi. The fittings kit is designed with 3 separate parts which include a black carbon-PEEK ferrule, a stainless steel triangular ferrule and a stainless steel nut. The carbon PEEK ferrule deforms when the stainless steel nut is tightened and the stainless steel triangular ferrule clamps onto the tubing at the same…
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) detection, expanded scouting options, large-sample injection, outlet valve, and column switching of up to 5 columns, for method development
Includes 100 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, and automated sample injection, for the purification of proteins
Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users. Column Details: Mode of chromatography: Hydrophilic interaction…
Cogent Bidentate C8™ HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a straight chain C8 hydrocarbon bonded directly to the Silica Hydride surface with two separate points of attachment. The manufacturing of the Silica makes these columns virtually indestructible.
The BLC-30 from Buck Scientific offers analytical versatility at an excellent price. The BLC-30 HPLC is an isocratic system with a variable wavelength detector. The BLC-30 Series Analytical HPLC systems use a high-quality, precision monochromator based spectrophotometer for a full range of wavelengths from 190-800nm for selective analyses integrated into a complete LC package. The 20-series is easily upgraded to a gradient Sy…
We offer the Sanwatsusho degasser with our HPLC line for those who are dealing with samples which are aerated. Specifications: Max flow rate 7ml/min Residual dissolved oxygen: 0.8 ppm maximum at flow rate of 1 ml/min. Pressure Loss - 0.6 kPa - (0.006 kg/cm2 or 0.086 psi) at flow rate of 1 ml/minute Internal Volume : 10ml
Filter Fluorescence Detector For HPLC - Customer to select wavelength. The new DFL-10 HPLC Fluorescence detector provides the sensitivity and the specificity of fluorescence detection at a price consistent with most low cost UV detectors.The DFL-10 can be configured with a choice of lamps covering the UV and Visible regions of the spectrum. Choices include Deuterium (at an additional cost with a recommended expanded range pho…
Fraction Collector - 116 tube capacity for 10 to 16 mm tubes. Our fraction collector can be operated as a simple fraction collector, where each fraction is the same size and the fraction size is set in terms of time, drops, or external events (like counts from a pump). A simple command entered from the membrane keypad enables the internal peak detector. This allows you to collect only those peaks of interest from the chromato…
We have found the Schambeck Refractive Index Detector to work very well with our HPLCs. Our fraction collector can be operated as a simple fraction collector, where each fraction is the same size and the fraction size is set in terms of time, drops, or external events (like counts from a pump). A simple command entered from the membrane keypad enables the internal peak detector. This allows you to collect only those peaks of…
Spark Holland OPTIMAS 84 Position Autosampler. OPTIMAS features our proven PASA injection concept, combining robust injection with high precision and accuracy. With its three injection modes including zero sample loss injection, OPTIMAS rivals top-class autosampler performance, while challenging middle-class autosampler prices! Features: Reliable PASA injection technology Superior price/ performance ratio Sample c…
The SEDEX Model 75 provides the option of 4 new nebulizers and a redesigned evaporation tube. These changes allow you to use a lower temperature to evaporate the mobile phase, this provides a more uniform droplet size, maximizing the sensitivity and repeatability. Enhanced ease of operation The SEDEX Model 75 is designed to be very easy to use. The operator needs to set only three controls, the gain, the zero and the evaporati…
Use Thermo Scientific™ GlycanPac™ AXH-1 LC Columns for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules.This high-performance, silica-based high-performance liquid chromatography (HPLC) column provides simultaneous separation of glycans by charge, size and polarity. Achieve high-resolution and high-throughput analysis with unique selectivity for biologi…
Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical da…
For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. Featuring a lower hydrophobicity than C18 or C8 columns, Hypersil GOLD C4 columns provide outstanding sensitivity and high efficiency.
