HTS Assay Kits Products & Reviews

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes.

BellBrook Labs AptaFluor™ SAH Methyltransferase Assay

BellBrook Labs Transcreener cGAMP cGAS Assay

BellBrook Labs Transcreener® GDP GTPase Assays

The Mosaic ELISA Human Cytokine Panel 1 is an excellent tool for the detection of 8 different cytokines in the same sample. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments. Cytokines are pleiotropic extracellular signaling molecules that influence myriad physiological and pathological processes. Released…

EMD Millipore's hERG membrane preparations are crude membrane preparations made from HEK293 stable recombinant cell lines, which are ideal HTS tools for screening antagonists against the hERG channel. The membrane preparations exhibit a Kd of 5.2 nM for [3H]-Astemizole. With 10 μg/well hERG Membrane Prep and 3.0 nM [3H]-Astemizole, a greater than 4-fold signal-to-background ratio was obtained.

EMD Millipore's cAMP HTS immunoassay kit, 384 well is a competitive immunoassay for quantitative chemiluminescence-based detection of cAMP in cell lysates and supernatants, which has been optimized for use in high throughput screening applications. The kit provides two ready to use 384 well pre-coated assay microplates and all reagents required to perform the assay.

The ProteoPlex™ 16-Well Human Cytokine Array Kit is designed for multiplex detection and measurement of 12 important human cytokines in parallel from up to 15 experimental samples. The combination of advanced surface chemistry, well-characterized capture agents, and a highly-sensitive fluorescence-based detection system provides a robust method for comparing relative cytokine abundance or cytokine quantification in serum sampl…

An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…

A rapid and sensitive homogeneous assay for cortisol assessmentThe HTRF® cortisol assay can be used for the quantification of cortisol in rodent serum, or directly on cells. This assay has also been validated for 11-beta HSD1 screening on whole cells or microsomes.The assay requires only 2 reagent additions, followed by an incubation period of 2 hours at room temperature. It has a detection limit of 70 pg/mL (e.g. 2 fmol/well…

A straightforward tool for biomolecular labeling with Europium cryptateThe Cryptate labeling kit includes all the necessary materials to allow rapid Cryptate labeling of various biomolecules and purification. The labeling reagent Eu3+-TBP-NHS Cryptate is suitable for labeling peptides, proteins, and oligonucleotides, as well as small molecules containing amino groups (N-terminus or lysine residues). The labeling procedure requ…

A range of HTRF® conjugates for assay developmentHTRF®  toolbox assembles a panel of detecting reagents such as Streptavidin-XLlent!, anti-tag antibodies like Eu-Cryptate anti-GST, 6HIS, DNP and anti species conjugated antibodies offering many possibilities for the study of molecular mechanisms. All reagents are available as Cryptate-Europium or XL665 (the two HTRF tracers) conjugates, to allow easy assay development. These re…

Streamlined single plate protocols including all stimulation and cytokine quantification.The HTRFâ cytokine kit line includes TNFa, IFNg, IL1b, IL6 and IL8 assays. These assays feature no separation steps, a high sensitivity and dynamics, and no use of isotopes. Procedures are performed on a single plate with simultaneous cell stimulation and cytokine detection. They can all be run under a similar experimental procedure stream…

A new secondary messenger for Gq coupled receptor investigation.Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity. This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stab…

Ready-to-use, far-red fixed cell counterstain

Green Flourescent Caspase-3/7 Apoptosis assay for live cell assays. Analysis by flow cytometry, microscopy or fluorescence microplate

Red Flourescent Pan Caspase Apoptosis assay for cell based assays

Green Flourescent Caspase-3/7 Apoptosis assay for live cell assays. Analysis by flow cytometry, microscopy or fluorescence microplate

Green Flourescent Poly Caspase Cell death assay for cell based assays

Green Flourescent Pan Caspase Apoptosis assay for live cell assays

Green Flourescent Caspase 9 Apoptosis assay for cell based assays

For researchers who want to do cell cycle research at their benchtop, Guava Cell Cycle assay can be used for measuring G0/G1, S, and G2/M phase distributions. Based on the established method of whole-cell staining with propidium iodide (PI), Guava's cell cycle assay is easy to learn. Guava Cell Cycle software includes markers that can be set for quick assessment of G0/G1, S, and G2/M phase cell cycle percentages as data is bei…

Apoptosis, or programmed cell death, is a highly regulated pathway that is important in normal developmental processes as well as many diseases. Cells undergoing apoptosis are identifiable by a number of characteristics, including changes in mitochonrial membrane potential, transport of phosphatidylserine (PS) to the membrane surface, activation of caspase proteases, and DNA fragmentation in the nucleus. Guava offers four apop…

Guava Technologies' ViaCount assay is revolutionizing the way cell counting and viability assessment are done in today's laboratories. Fast, automated, and highly reproducible, the Guava ViaCount assay addresses many of the difficulties of conventional cell counting and viability assessment methods. The Guava ViaCount assay provides: Cell counts up to ten times faster than manual counting, in a convenient turnkey assay.…

Cisbio's HTRF® KinEASE™ platform has expanded to include a universal assay for tyrosine kinases. The HTRF KinEASE TK assay is based on our patented HTRF technology and uses a unique tyrosine peptide substrate in combination with a Europium-labeled monoclonal antibody. The HTRF KinEASE™ TK assay has been validated on 59 tyrosine kinases to-date including both receptor and cytoplasmic TKs. A proprietary substrate stabilizing buf…