Assay Assembly Products & Reviews

25

Assay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.

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Transcreener® cGAMP cGAS Assay

BellBrook Labs

The Transcreener® cGAMP cGAS Assay Kit offers mix-and-read fluorescent detection of cGAMP—the product of cGAS activity—with no coupling enzymes required. Available in FP or TR‑FRET readouts (far-red tracers minimize interference), it’s HTS-ready (Z’ ≥ 0.7), automation-compatible, and validated on 96‑, 384‑, and 1536‑well formats for enzyme kinetics, SAR, and inhibitor screening.

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Biotinylated Human TNF-alpha Protein, His,Avitagâ„¢, active trimer (MALS verified)

ACROBiosystems

Biotinylated Human TNF-alpha, His, Avitag (TNA-H82E3) is expressed from human 293 cells (HEK293). It carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitagâ„¢), and has a calculated MW of 20.0 kDa. The protein migrates as 21 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

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Human VEGF165 Protein, His Tag

ACROBiosystems

Human VEGF165, His Tag (VE5-H5248) is expressed from human 293 cells (HEK293). It carries a polyhistidine tag at the N-terminus, and has a calculated MW of 21.1 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

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Biotinylated Human VEGF165 Protein, His,Avitagâ„¢

ACROBiosystems

Biotinylated Human VEGF165, His,Avitag(VE5-H82Q0) is expressed from human 293 cells (HEK293). It carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitagâ„¢), and has a calculated MW of 22.4 kDa (monomer). The protein migrates as 25-33 kDa (monomer) under reducing (R) condition, and 42-55 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

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PTEN, active

Merck

PTEN hydrolyzes phosphate at the 3 position on the inositol ring of PtdIns(3,4,5)P3, and Ins(1,3,4,5)P4. Although PTEN has the consensus sequence of a protein tyrosine phosphatase, it dephosphorylates p-nitrophenylphosphate and other synthetic and protein substrates poorly. The greatest catalytic activity has been observed with the highly negatively charged, multiply phosphorylated polymer of (Glu-Tyr)n.

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Staurosporine

Merck

Suitable for use as a potent inhibitor of PKC and other protein kinases in vitro and in cultured cells.

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Venom Discovery Array (VDA)

Venomtech Limited

The Venom Discovery Array (VDA) is a naturally compressed, hit-to-tool screening library based on a 96-well automation compatible array. These VDA's can be customised to meet target specific needs, such as the 48 phylogenetically diverse tarantula species to target ion channels or snakes for cancer research amonst others.For further information please click on the company website link.

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