Products & Reviews

Single-Use Ligands for High-Resolution lmaging in Live Cells

Bioluminescent Assays to Detect NAD(P)+ and NAD(P)H Levels in Cells Homogeneous, single-reagent-addition assays Easily adapted for high throughput screening formats

The RealTime-Glo™ MT Cell Viabiilty Assay measures viability of cells in a non-lytic, real-time live-cell format, saving time, cells and reagent costs.

Detect and quantify any tagged protein with a simple, luminescent signal.

Measure neonatal Fc receptor binding  

The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at l…

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…

Experience improved PCR performance with GoTaq® DNA Polymerase products. GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and in some cases superior to that of standard Taq DNA polymerase. GoTaq® DNA Polymerase comes in a variety of formulations designed to give you maximum flexibility, control and convenience.

The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultur…

The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA.   The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows…

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…

Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

The Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The CytoTox 96® Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that result…

GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased re…

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…

Western Blot Incubation Boxes are suitable for incubating near-infrared fluorescent or chemiluminescent Western blots or gels.

The Odyssey® CLx Infrared Imaging System gives you accurate, reproducible quantitative Western blotting results with near-infrared fluorescence.

NewBlot Western Blotting Stripping Buffers conserve precious samples by reusing the same blot and you save money.

The WesternSure Pen is used to annotate visible protein ladders prior to chemiluminescent Western blot detection.  The pen is optimized for detection using the C-DiGit® Blot Scanner or the Odyssey® Fc Imaging System.

Empiria Studio Software is the ideal software for labs that want workflow-based analysis to ensure consistent results for all researchers. Researchers of all experience levels can easily determine target protein expression, analyze validated antibody experiments, compare replicates, perform statistical analysis, share results, and much more—all in one intuitive application.  

Imaging probes conjugated to IRDye 800CW and IRDye 700DX have propelled more than 20 Phase I and Phase II clinical trials worldwide.

A research-grade rotator and features the ability to use rotating ring-disk and cylinder electrodes.

The SENTERRA II defines a new level of spectroscopic performance and user friendliness in the class of compact Raman microscopes. SENTERRA II is designed to deliver excellent sensitivity combined with high spectral and imaging performance. Therefore the SENTERRA II is a powerful platform for advanced research applications. Due to its high degree of automation, compact size and efficient workflow the SENTERRA II is likewise an…

The VERTEX 80 and the VERTEX 80v vacuum FTIR spectrometers are based on the actively aligned UltraScan™ interferometer, which provides PEAK spectral resolution.   The precise linear air bearing scanner and PEAK quality optics guarantees the ultimate sensitivity and stability. The VERTEX 80v is an evacuated optics bench that can eliminate atmospheric moisture absorptions for ultimate sensitivity and stability; enabling de…

The FT-Raman module RAM II can be coupled to Bruker's VERTEX Series multi range FTIR spectrometers, utilizing the spectrometer’s one input and one output port. Switching between the infrared and Raman is fully software controlled and combines fast and easy sample handling for both measurement modes.   For utmost suppression of fluorescence the RAM II module is equipped with standard 1064 nm excitation. The module can be…