Real-Time PCR (qPCR) Mastermixes and Kits Products & Reviews

RNase Zap ® RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNase Zap ® Solution onto the surface to be decontaminated and rinse it off with RNase-free water.

PowerUp™ SYBR ® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

igScript™ one step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity, and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions. igScript™ Reverse Transcriptase is a recombinant MMLV r…

Choice Taq Mastermix is a 2X reaction mixture. It is ready-to-go and it only requires addition of template, primers, and water.

qPCRBIO SyGreen® Mix combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive, and reproducible real-time PCR.

Our universal probe kit is designed to give superior sensitivity and specificity in all probe-based real-time PCR assays, including TaqMan, Scorpions and molecular beacon probes. Whether your application is for a singleplex or multiplex expression study or for genotyping and allelic discrimination analyses, qPCRBIO Probe Blue Mix is the robust choice for all your probe-based real-time PCR needs.

The Mic ensures fast and highly accurate results. This real-time PCR cycler is bundled into a small 2kg cube so you can bring it anywhere.

Clara® Probe Mix is the newest solution for qPCRs. With clear and consistent results, this cutting-edge qPCR mix will streamline your real-time PCR workload no matter what the application.

FastStart Universal Probe Master (Rox) includes a novel reference dye, which enables its use without modification or adjustments to the specific instrument or protocol on all real-time PCR instruments requiring normalization with ROX. This ready-to-use, 2x-concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-s…

Thermo Scientific ABsolute Fast QPCR Master Mix delivers highly reproducible, sensitive and specific detection of DNA and cDNA targets, using sequence-specific probes, under fast thermal cycling conditions. Features The ABsolute Fast QPCR Master Mix is an addition to the ABsolute Blue QPCR & Verso QRT-PCR product portfolio. Like both of these mixes, the ABsolute Fast QPCR Master Mix contains a pre-blended inert blue dye which…

Solaris qPCR Master Mix has been developed in conjunction with Solaris qPCR Gene Expression Assays for quantification of DNA and cDNA. Optimal qPCR results using probe detection chemistries can be achieved by using this same system of qPCR products. Optimized Solaris qPCR Master Mixes have been developed to ensure the best possible Solaris assay performance. In addition to enhanced performance, these optimized reagents offer…

Brilliant II SYBR qPCR and QRT-PCR reagents provide improved sensitivity and detection while ensuring reproducible quantification at low target concentrations. Compared to other commercially available qPCR reagents, Brilliant II SYBR qPCR and QRT-PCR reagents have earlier threshold cycle (Ct) detection at high and low template concentrations.

Brilliant II QPCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. For faster experiments with earlier Ct's and greater reproducibility, Agilent recommends Brilliant III Ultra-Fast master mixes.

Appropriate for COVID-19 detection.

GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased re…

50 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq DNA polymerase

Real-time fluorescent DNA detection

100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.   Features:…

100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO

Experimentally validated PCR primer and probe assays for gene expression, copy number variation, and mutation detection analysis for real-time PCR and Droplet Digital PCR.

250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2

BHQ® (Black Hole Quencher®)-labeled Scorpions primers for PCR analysis are unlike dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end. Scorpions primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR.  Similar to Molec…

Components : Tks Gflex DNA Polymerase 250 U 2 X Gflex PCR Buffer (Mg2+, dNTP plus) 1 ml x 5 Mg2+ conc. (2 X) 2 mM dNTP conc. (2 X) ea. 400 μM