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Multiplex protein analysis in human aqueous humor, vitreous humor, tears

Multiplex protein analysis in human aqueous humor, vitreous humor, tears

JC-1 is a membrane permeable dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes coloras membrane potentials increase (over values of about 80-100 mV). When excited at 488 nm, the emission shift goes from 530 nm (monomeric JC-1) to 590 nm (aggregate JC-1), which are easily detected using filter…

eFluor 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. When it enters a cell, cellular esterases cleave the dye, resulting in a membrane-impermeable dye that fluoresces at ~520 nm. eFluor™ 514 Calcium Sensor Dyeis a commonly used dy…

DRAQ5, an anthraquinone dye with high affinity for double-stranded DNA, is a membrane-permeable dye that can label live or fixed/dead cells. The dye is used to distinguish nucleated and non-nucleated cells in flow cytometry, to report nuclear DNA content for ploidy and cell cycle analysis and as a nuclear counterstain nuclei in fluorescent microscopy.DRAQ5 can be excited by wavelengths from 488 to 647 nm. For microscopy/imagin…

The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. PMA and ionomycin treatment can induce activation of many cell types to produce cytokines. Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus. The Cell Stimulation Cocktail (plus protein transp…

The Phytohemagglutinin-L (PHA-L) Solution (500X) is a ready-to-use solution of PHA-L in aqueous buffer. PHA is a mixture of lectins isolated from the red kidney bean, Phaseolus vulgaris. PHA-L is the fraction of PHA that exhibits strong leukoagglutinating and mitogenic activity, but very little erythroagglutinating activity. This reagent is intended for use in in vitro activation of human and mouse leukocytes.

The Concanavalin A (Con A) Solution (500X) is a ready-to-use solution of Con A in aqueous buffer. Con A, isolated from the Jack bean, Canavlia ensiformis, is a lectin that binds alpha-D-glucose and alpha-D-mannose moieties found in various glycoproteins, glycolipids, and sugars and is a potent leukocyte mitogen. This reagent is intended for use in in vitro activation of human and mouse leukocytes.

CellEvent Caspase-3/7 Green Ready Flow Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteExceptionally bright green fluorescen…

The Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent…

The Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent…

The Click-iT Plus EdU Pacific Blue Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalen…

The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…

The Click-iT Plus EdU Alexa Fluor 594 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…

CellTrace CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace CFSE dye. Using this dye, eight or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of dist…

CellTrace Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Violet dye. Using this dye, ten or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of di…

CellTrace Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Far Red dye. Using this dye, six or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of…

CellTrace Yellow Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Yellow dye. Using this dye, six or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of di…

CellTrace Blue Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Blue dye. Using this dye, five generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of distinct gene…

The LIVE/DEAD BacLight Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of micros…

The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO™ 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. SYTO 9 stain generally labels all yeast in a population, with or without intact membranes. Propidium iodide penetrates only…

The LIVE/DEAD BacLight Bacterial Viability Kit for microscopy and quantitative assays provides a convenient and easy-to-use solution for monitoring the viability of bacterial populations based on the membrane integrity of the cell. Cells with a compromised membrane that are considered to be dead or dying will stain red, whereas cells with an intact membrane will stain green.

The LIVE/DEAD Fixable Blue Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…

The LIVE/DEAD Fixable Violet Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…

The LIVE/DEAD Fixable Aqua Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Aqua Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…