Identification and optimization of the chemical, series of chemicals or bio-molecule that will interact with the target using impartial, expert opinion about the best equipment, services and the latest techniques
Thermo Scientific Online SPE Products have been designed to clean-up and concentrate target analytes in minutes. The products are available in different formats and utilize popular HyperSep SPE materials such as the HyperSep™ Retain polymeric range of materials and HyperSep Hypercarb™ for polar analyte retention.
A successful packing material from the start: LiChrosorb® is one of the most successful and reliable packing materials used in HPLC for more than 25 years and documented in literature in the form of several thousand applications. The totally porous irregular particles are finely classified in the 5, 7 and 10 µm range. LiChrosorb® offers the complete programme of non-polar derivatives (RP-8, RP-18, RP-select B) polar derivativ…
Symbiosis™ Pharma is Spark Holland's unique solution for integrated online SPE-LC-MS automation (XLC-MS). The heart of the matter in XLC-MS, is where the autosampler injects at a controlled temperature, raw bio-fluids directly on a fresh disposable SPE cartridge. The small particle sorbent assures a clean extract, a very high sensitivity and reliable results. Online elution to the LC-MS cuts the number of sample prep steps in…
Hitachi’s L-2300 Column Oven incorporates new technology that provides superior temperature stability and reduces thermal gradients resulting in greatly enhanced performance. Peltier heating and cooling allow short equilibration times. In addition, solvent is preheated prior to reaching the column which results in stable temperature inside the column. System connectivity ensures that sample injections are made only when temper…
Spherical silica carrier for constant results on high level: LiChrospher® are reliable and versatile traditionally produced spherical silica carrier. LiChrospher® silica carrier are available with different modifications. For the LiChrospher® RP-phases a selectivity and performance guarantee makes sure that these parameters remain constant form batch to batch and from year to year. The polar modified phases LiChrospher® CN, L…
Hitachi's LaChrom Elite HPLC and Sensivate Elite PCR deliver turn-key analysis kits for a variety of compounds. Carbamate pesticides and glyphosate herbicide kits are available, which achieve low-level detection with high selectivity. Derivatization kits with ninhydrin or o-phthaldehyde offer cost effective solutions for the analysis of Amino Acids via PCR. The kits include analytical columns, reagents, buffers, and instructio…
The Hitachi F-7000 Fluorescence Spectrophotometer offer superior performance, functionality, and reliability. Designed to conserve benchspace (620 mm W x 520 mm D x 300 H mm), the F-7000's Ultra-High Wavelength accuracy: (S/N:800) and High-Speed Scanning (60,000 nm/min) provide unparalleled performance. • Bandpass: 1, 2.5, 5.0, 10, 20nm (EX and EM) • Wavelength Scan Speed: 30, 60, 240, 1200, 2400, 12000, 30000 and 60000nm/min…
LiChrospher® CN is a versatile HPLC sorbent based on spherical silica particles possessing both polar and hydrophobic properties. LiChrospher® CN may be used as weakly hydrophobic sorbent in reversed phase chromatography or as polar sorbent in normal phase chromatography.
LiChrospher® DIOL is a versatile HPLC sorbent based on spherical silica particles possessing both polar and hydrophobic properties. LiChrospher® DIOL may be used as weakly hydrophobic sorbent in reversed phase chromatography with some limitations or as polar sorbent in normal phase chromatography. LiChrospher® DIOL is also suitable for exclusion chromatography.
A versatile sorbent for both reversed phase and straight phase chromatography: LiChrospher® NH2 is a versatile HPLC sorbent based on spherical silica particles possessing both polar and hydrophobic properties. LiChrospher® NH2 may be used for normal phase chromatography, reversed phase chromatography and for ion exchange chromatography. Typical applications are the separation of carbohydrates (mono-, di- and oilgosaccharides)…
A new member of the HyPURITY® family introduced at Pittcon 2003, HyPURITY AQUASTAR™ columns are a new silica-based polar end-capped C18 which is designed to offer superior retention of polar compounds and increased sensitivity in 100% aqueous conditions. The robust nature of the bonded phase results in a silica-based column that is unsurpassed for LC and LC/MS applications. This next generation column also offers enhanced perf…
LiChrospher® PAH is a highly efficient and selective HPLC column especially designed for the high resolution separation of 16 PAH (polycyclic aromatic hydrocarbons) according to EPA 610 and 550 + benzo(e)pyrene + perylene. LiChrospher® PAH is based on a special modified RP-18 silica gel to achieve highly resolved PAH separations. LiChrospher® PAH can be used for both, the isocratic separation of 6 PAH according to the German…
The Vision diode array spectrophotometer offers 16 predefined methodologies for nucleic acid quantification (DNA, RNA, and oligonucleotides), protein assays (BCA, Biuret, Bradford, and Lowry) and for cell culture density measurements. Additionally, the Vision can accomodate up to 90 custom protocols. The visualization of the nucleic acid scan with the full graphic display is particularly useful, especially for RNA samples wh…
The ASIC1a gene encodes the pore-forming subunit of a proton-gated, non-selective cation channel. ASIC1a channels expressed in neurons are therapeutic targets for treatment of pain and depression. Synonyms: Acid-sensing (proton-gated) ion channel Host cell: CHO-K1, division-arrested Gene name: ACCN2 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 ce…
The ACCN1 gene encodes the pore-forming subunit of a proton-gated, non-selective cation channel. ASIC2a channels expressed in neurons are therapeutic targets for treatment of pain. Synonyms: Acid-sensing (proton-gated) ion channel Host cell: CHO-K1, division-arrested Gene name: ACCN1 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growt…
The ACCN31 gene encodes the pore-forming subunit of a proton-gated, non-selective cation channel. ASIC3 channels expressed in neurons are therapeutic targets for treatment of pain. Synonyms: Acid-sensing (proton-gated) ion channel Host cell: CHO-K1, division-arrested Gene name: ACCN3 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growt…
Human Cav2.2/β3/α2δ1 ion channel-expressing, division-arrested cells (CHO-K1). Cat#: CT4159. Three vials of Cyropreserved irradiated cells, 2x10E6 cells/vial (6x10E6 cells total). Platform validation: PatchXpress®
The human CACNA1B gene encodes the pore-forming subunit of voltage-gated N-type Ca2+ channels. Co-expression with the β3 and α2δ1 auxiliary subunits recapitulates function in heterologous expression (CHO cells). Cav2.2 channels, widely distributed in neurons, are therapeutic targets in neuropathic and inflammatory pain. Synonyms: N-type calcium channel Host cell: CHO-K1, division-arrested Gene name: CACNA1B/CACNB3/CACNA2D1 My…
The HCN4 gene encodes the pore-forming subunit of a hyperpolarization-gated, cyclic nucleotide-sensitive, non-selective cation channel. HCN4 channels expressed in the heart contribute to the pacemaker current (Ih) and are therapeutic targets for treatment of cardiac ischemia and angina. Synonyms: hyperpolarization-activated, cyclic nucleotide-gated channel Host cell: CHO-K1, division-arrested Gene name: HCN4 Mycoplasma status…
The human KCNA3 gene encodes the pore-forming subunit of a voltage-gated potassium channel. Kv1.3 channels expressed in lymphocytes are therapeutic targets in autoimmune diseases. Synonyms: hKv1.3 Host cell: CHO-K1, division-arrested Gene name: KCNA3 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth…
The human KCNQ1 gene encodes the pore-forming subunit of a voltage-gated potassium channel. Co-expression with minK, an auxiliary subunit, recapitulates native function in heterologous expression (CHO cells). KvLQT1/minK channels expressed in the heart are safety targets in congenital and acquired long QT syndromes. Synonyms: Kv7.1/minK Host cell: CHO-K1, division-arrested Gene name: KCNQ1/KCNE1 Mycoplasma status: Negative (M…
The human KCNQ4 gene encodes the pore-forming subunit of a voltage-gated potassium channel. KCNQ4 channels expressed in CNS neurons are therapeutic targets in epilepsy. Synonyms: Kv7.4 Host cell: CHO-K1, division-arrested Gene name: KCNQ4 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth characteris…
The KCNH2 gene encodes the pore-forming subunit of a voltage-gated potassium channel. HERG channels expressed in cardiac muscle cells are safety targets; hERG inhibition has been linked to drug induced prolongation of the QT interval and torsade de pointes (a potentially lethal cardiac arrhythmia). Synonyms: Kv11.1 Host cell: CHO-K1, division-arrested Gene name: KCNH2 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Thr…
The human SCN1A gene encodes the pore-forming subunit of voltage-gated type-1 Na+ channels. Neuronal Nav1.1 channels are therapeutic targets in seizure, pain, and stroke. Synonyms: brain type-1 sodium channel Host cell: CHO-K1 Gene name: SCN1A Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cyropreserved irradiated cells, 2 x 106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth char…
The human Nav1.2 gene encodes the pore-forming subunit of voltage-gated type-2 Na+ channels. Neuronal Nav1.2 channels are therapeutic targets in seizure, pain, and stroke. Synonyms: brain type-2 sodium channel Host cell: CHO-K1 Gene name: SCN2A Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cyropreserved irradiated cells, 2 x 106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth cha…
