Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
Cellena® is a bioencapsulation portable platform able to encapsulate high molecular weight compounds, microorganisms and cells in homogeneus particles of predictable and controlable size. Designed by Ingeniatrics, it has been specifically developed for biotechnological research. Its performance is based on Flow Focusing® technology. By means of this technology, the elements to be encapsulated are immobilized within a semiperme…
The Clarisolve® depth filter is a novel clarification tool with a gradient density structure specifically designed to the particle size distributions of pretreated feed streams. Offering improved volumetric capacity and reduced turbidity over current available depth filters, the clarification step of pre-treated feeds can be processed in a significantly reduced footprint without the need for a secondary stage of clarification…
Support transient transfection, growth, and expression of a variety of recombinant proteins in HEK293 cells with Thermo Scientific™ HyCell TransFx-H Media, a high-performining, animal-derived component-free cell culture media. HyCell TransFx-H media is a hydrolysate-free and regulatory-friendly Thermo Scientific ADCF™ cell culture media. Its versatility allows quick adaptation and supports excellent transfection, high viable…
Support transient transfection, growth, and expression of a variety of recombinant proteins in CHO cells with Thermo Scientific™ HyCell TransFx-C Media, a high-performing, animal-derived component-free cell culture media. HyCell TransFx-C media is a hydrolysate-free and regulatory-friendly Thermo Scientific ADCF™ cell culture media. Its versatility allows quick adaptation and supports excellent transfection, high viable cell…
The PowerViral™ Environmental RNA/DNA Isolation Kit is designed for fast and easy purification of viral and bacterial total nucleic acids from samples high in PCR inhibitors; including waste water, stool, biosolids, and gut material. Patented Inhibitor Removal Technology® (IRT) ensures complete removal of the inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compoun…
Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The Phire® Animal Tissue Direct PCR Kit employs Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA-binding domain that enhances the processivity of the polymerase. Phire Hot Start II DNA Polymerase also exhibits extreme…
Thermo Scientific Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood. This extremely accurate proofreading DNA polymerase makes the Phusion B…
If there is DNA to detect, the Plexor® HY System will show it. Total human DNA. Total male DNA. The power to see it all in a single assay. The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors…
Efficient siRNA or microRNA transfection. To attain efficient and reliable siRNA or microRNA transfection, we offer DharmaFECT transfection reagents. Thermo Scientific DharmaFECT 1 is our most all-purpose transfection reagent formulation; demonstrating efficient, low-toxicity delivery to over 35 cell types. DharmaFECT 1 achieves effective transfection of siRNA and microRNA reagents at low concentrations and with low cytotoxic…
Pre-designed, gene-specific probe and primer pairs for repeatable, sensitive and gene specific RT-qPCR quantification for human and mouse protein-coding genes and long noncoding RNAs (lncRNAs). Thermo Scientific Solaris qPCR Gene Expression Assays are pre-designed, gene specific probe and primer pairs that utilize minor groove binder (MGB) and Superbase technologies to deliver repeatable, sensitive and gene specific RT-qPCR (…
SmartRNAplex miRNA Assays are your benchtop solution for miRNA profiling, using a familiar protocol, and simple acquisition software. SmartRNAplex microRNA Assays use proprietary Firefly™ technology, which exploits uniquely encoded hydrogel particles developed by innovators at Firefly BioWorks. The Firefly™ hydrogel particles are hydrophilic, bio-inert, flexible and porous, allowing target molecules to diffuse and bind in a…
The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase. Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…
Introducing Diagenode’s latest innovation – a superior method for extracting DNA from FFPE samples. The FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by tradtional methods or can be sheared with the Bioruptor® Pico ultrasonicator for downstream next g…
The AttoPhos® AP Fluorescent Substrate System provides a highly sensitive fluorescent alkaline phosphatase substrate. The system includes AttoPhos® Substrate, AttoPhos® Buffer and Calibration Solution. AttoPhos® Substrate (2′-[2-benzothiazoyl]-6′-hydroxybenzothiazole phosphate [BBTP]) is cleaved by alkaline phosphatase to produce inorganic phosphate (Pi) and the alcohol, 2′-[2-benzothiazoyl]-6´-hydroxybenzothiazole (BB…
The TnT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix. The TnT® Quick Coupled Transcription/Translation System is ava…
The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. Kazusa DNA Research Institute (KDRI) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. KDRI is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Uni…
The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression. These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli. Transformation efficiencies are greater than 108 cfu/μg, similar to other highly competent cells. The single step cells are available in single transformation size (50μl). KRX a…
For standard cloning experiments competent cells are available in two formats: high efficiency at greater than 108cfu/μg for cloning (JM109, HB101), and subcloning efficiency at greater than 107cfu/μg (HB101). JM109 cells have the additional feature of blue/white screening for recombinants. For protein expression, JM109(DE3), BL21(DE3) or KRX competent cells can be used. The Single Step (KRX) Competent Cells are designed fo…
Diamond™ Nucleic Acid Dye is a safe, sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels. Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system. Significantly less mutagenic than traditional ethidium bromide, as proven by Ames…
The BenchTop DNA Markers, supplied in a stabilizing solution of 1X Blue/Orange Loading Dye, come in a variety of different sizes to meet your DNA electrophoresis needs.
Promega has partnered with ATCC to offer unparalleled STR testing service for human cell line authentication. This service provides easy access to proven and comprehensive validation techniques. Promega's proprietary PowerPlex® and GenePrint® STR technology coupled with ATCC's considerable experience profiling human cell lines and extensive internal assets around STR profiling data interpretation is particularly r…
High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits: Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…
GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase. This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…
Lockable ICRplus Settle Plates – For more than just safe transportation The new ICRplus Settle Plates combine the high standard of ICR Settle Plates with a novel two way closure system to increase safety and reliability for active and passive Air Monitoring. Like ICR Settle Plates, the ICRplus Settle Plates are produced under aseptic conditions, gamma-irradiated and triple-bagged, making them optimal for passive air sampling…
QUAD® Sutter Instrument 4-axis motorized micromanipulator, is easy-to use and features four independent axes. Three axes provide the X, Y, Z-orthogonal motion typical of most motorized micromanipulators. In the QUAD, Sutter introduces a true fourth axis to move the electrode coaxially at exactly the desired angle of approach. The fourth axis also significantly extends the range of travel for the system. The QUAD is very…
