Chromolith ® columns have shown great potential and superiority in comparison to standard silica. In contrast to conventional, packed-particle columns, wide pore (300 Å) monolithic silica columns are made of a single continuous-bed rod of high purity porous silica that is then bonded with C18, C8, C4, Epoxy, and Protein A depending on the use of the column.
Accurate Photometric analysis for Water, Environmental, Food and Beverage Testing
The T7 Endonuclease Detection Assay is a well-known method for detecting genome editing events from CRISPR, Zinc-finger nuclease, and TALEN gene targeting. Originally identified from Escherichia coli bacteriophage, the T7 endonuclease can cleave mismatched heteroduplex DNA, Holliday junctions, branched DNA, and cruciform DNA.
All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids for use with monocots and dicots.
Spectroquant ® tests and kits for accurate, precise and consistent results
CRISPR/Cas9-mediated recombineering is the most powerful bacterial genome engineering method to date. In addition, Cas9-mediated recombineering overcomes the dependence on a second recombination step, avoids the creation of destabilizing scar sites, can be used in multiplexing, and is less time-consuming than previous protocols.
This Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.
Universal negative control CRISPRs have been designed not to recognize any sequence in the human, mouse or rat genome.
Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA ® synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.
An easy-to-use, modular platform to co-culture different cell types in discrete 3D and 2D compartments.
Exclusive human or mouse whole genome arrayed libraries developed with the Wellcome Sanger institute
Lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology.
Pooled and Arrayed Genome-Wide LentiORF Libraries for High-Throughput Screens or Targeted Experiments
Recombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments
Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.
Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.
Trusted TRC (The RNAi Consortium) content with shRNA targting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma
We have applied CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development.
CompoZr ® ZFN (zinc finger nuclease) technology was used to insert the sequence for fluorescent proteins adjacent to endogenous genes to create reporter cell lines for compound screening
