Agilent Technologies UHPLC and HPLC Products & Reviews

The 1290 Infinity III Variable Wavelength Detector enables highest productivity with fast analysis at up to 240 Hz data rates. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.

The 1260 Infinity III Diode Array Detector WR offers multiple wavelength and full spectral detection up to a 120 Hz sampling rate. Maximum flexibility is guaranteed with a wide wavelength range of 190 to 950 nm with dual-lamp design, as well as the choice of 15 analytical, preparative, and SFC flow cells.

The 1260 Infinity III Evaporative Light Scattering Detector (ELSD), although targeted at analytical scale, can also be used with a flow splitter for preparative chromatography applications. It is highly recommended for compounds with little or no UV chromophore, since the detector technique evaporates the solvent and then measures the intensity of the scattered light, which is directly proportional to the sample concentration.

The 1260 Infinity II High-Temperature Evaporative Light Scattering Detector (HTELSD) is capable of detecting very low concentrations of high-molecular-weight polyolefins (polyethylene and polypropylene) at elevated temperatures. The need to analyze ultrahigh-molecular-weight polyethylene (UHMWPE) requires a new detection technique that offers a high level of baseline stability in order to reproducibly detect and permit accurat…

The 1260 Infinity III SFC Control Module is a carbon dioxide conditioning unit for supercritical fluid chromatography. This module pre- and postconditions CO2 for InfinityLab SFC solutions. CO2 preconditioning provides for unmatched stability and precision of retention times, and postconditioning enables trouble-free connection to mass spectrometers. 

The InfinityLab LC Companion acts as a mobile LC user interface to allow for remote control, monitoring, signal plotting, and diagnostics of Agilent 1260 and 1290 Infinity II LC systems. With the InfinityLab Companion, any mobile device - such as a tablet device or smartphone - with a compatible web browser can be turned into a mobile user interface to quickly check the status of your LC instrument.

Agilent Bio-Monolith Protein A LC columns are designed for analytical separation of most of  IgG subclasses (human and mouse). It delivers fast and accurate quantitation of mAb titer and purification of small amounts of mAb for further characterization. These monolithic column enables extremely fast separations to speed up method development time and decrease costs. They are compatible with HPLC and UHPLC systems.

The 1260 Infinity II Prime Online LC System is a process analysis system enabling automated process monitoring, providing real-time data for greater control and faster understanding of processes.

The 1260 Infinity II Online Sample Manager is an online sampling module that connects the analytical world with the process world. The module provides automated sample analysis via direct injections or retained samples from flow reactors, batch reactors, as well as upstream bioreactors and downstream purification devices.

The 1260 Infinity III Prime Bio LC System is a versatile HPLC instrument for bioseparations and offers outstanding functionality and operational convenience for biochromatography, and entry-level quaternary bio UHPLC at pressures up to 800 bar and flow rates up to 5 mL/min.

The 1290 Infinity II Bio High-Speed Pump is a binary UHPLC pump consisting of biocompatible material for use in biopharma and other applications utilizing high salt and extreme pH conditions. It uses high-pressure blending from up to two solvents at pressures up to 1300 bar and flow rates up to 5 mL/min.

The 1260 Infinity III Bio Flexible Pump is a UHPLC pump comprising biocompatible components, developed for biopharma applications and for other analyses requiring high-salt and extreme-pH conditions. This pump enables quaternary solvent delivery at pressures up to 800 bar and flow rates up to 5 mL/min.

The 1290 Infinity II Bio Multisampler, with its biocompatible sample flow path, is perfectly suited for biomolecule analysis, ensuring integrity of biomolecules and minimizing unwanted surface interaction. For temperature-sensitive samples, a compressor-based thermostatting device can be added.

AdvanceBio Peptide Plus columns are reversed phase, superficially porous particle LC columns optimized for the separation of target peptides, impurities, and post translational modifications.

The 1260 Infinity III Prime Analytical-Scale LC Purification System is a versatile preparative high-performance liquid chromatography (HPLC) instrument. This system offers the highest functionality and operational convenience for analytical-scale HPLC purification and entry-level quaternary UHPLC at pressures up to 800 bar and for flow rates up to 5 mL/min.

The 1260 Infinity III Prime Bio Analytical-Scale LC Purification System is a versatile preparative high-/ultrahigh-perfomance liquid chromatography (HPLC/UHPLC) system for bioseparations. The system offers outstanding functionality and operational convenience for bioanalytical HPLC and entry-level quaternary bio UHPLC at pressures up to 800 bar and flow rates up to 5 mL/min.

The 1260 Infinity III Bio-Inert Analytical-Scale LC Purification System is a dedicated solution for purifying large biomolecules in biological samples, including monoclonal antibodies and proteins.

The 1290 Infinity III Bio Analytical-Scale LC Purification System is an ultrahigh-performance liquid chromatography (UHPLC) system, configurable with a binary or quaternary pump and consisting of biocompatible material for use in biopharma (such as for critical quality attributes).

Agilent NISTmAb is a reference mAb standard that is intended for system suitability testing, establishing method, instrument, or site-to-site variability, and troubleshooting mAb CQA workflows.

Designed to withstand 100% aqueous conditions and increased retention for polar molecules, InfinityLab Poroshell 120 Aq-C18 columns are the modern and reliable choice for HPLC and UHPLC separations. Now master your polar molecules, especially under water.  

100Å pore size, high ligand density;  available in 1.0, 2.0,  3.0, 4.6 and 10 mm ids;  30 - 250 mm lengths. Pursuit columns are ideal for pharmaceutical applications using LC/MS, from analyzing lead compounds and biological samples in drug discovery, to implementing dependable analysis of raw materials and approved drugs in the QC process.  Build on the large 200A pore size silica, high liquid density delivers up to 40% faster…

The 1290 Infinity III Evaporative Light Scattering Detector (ELSD) is ideal for reproducible, sensitve detection of both volatile and nonvolatile compounds that cannot be detected by UV. A unique peltier cooled evaporator coupled with an original gas flow design allow subambient operation down to 10 °C. This delivers maximum sensitivity for compounds with significant volatility below 30 °C.

200A pore size;  available in 3.0 and 4.6  mm ids, and lengths from 50 - 250 mm. Pursuit columns are ideal for pharmaceutical applications using LC/MS, from analyzing lead compounds and biological samples in drug discovery, to implementing dependable analysis of raw materials and approved drugs in the QC process.  Build on the large 200A pore size silica, high liquid density delivers up to 40% faster separations without sacrif…

Agilent Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases.

"Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH - up to pH 11.5. Columns are best applied for separations of compounds that are either (1) basic and have little or no retention at low or intermediate pHs, (2) more stable or more soluble at high pH, or (3) basic and show poor peak shape at low or intermediate pH.…