ZAG DNA Analyzer System
The Agilent ZAG DNA Analyzer system is a high throughput parallel capillary electrophoresis instrument designed for the qualitative analysis of DNA fragments.
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Great result.
Forensic
This product is worthy of its cost. Very easy to use and its results are very accurate.
Review Date: 16 Jun 2021 | Agilent Technologies
DNA fragment analysis is a crucial procedure in many molecular biology facilities. The Agilent ZAG DNA Analyzer system streamlines the large-scale analysis of DNA fragments, providing researchers with an intuitive, high-throughput alternative to agarose gel electrophoresis.
The ZAG allows the loading of up to nine, 96-well sample trays for unattended operation. This capacity enables the analysis of up to 48, 96-well sample trays in a 24-hour period. All separations, regardless of kit, are completed in under an hour – some in as few as 20 minutes. With kits for sizing DNA fragments from 35 bp to as large as 20,000 bp, the ZAG streamlines any fragment analysis workflow, from PCR products to microsatellite analysis.
Features & benefits:
- High-throughput analysis – Analyze more than 4,600 samples in 24 hours
- Broad sizing range – Size DNA fragments from 35 bp to 20,000 bp with diverse reagent kit offerings
- High separation resolution – Accurate sizing is crucial for confident fragment analysis. The ZAG achieves 3 bp resolution for DNA fragments <300 bp
Brochures
Agilent automated electrophoresis portfolio brochure
Whether performing low- or ultra-high-throughput testing, Agilent Technologies' automated electrophoresis instrument portfolio delivers reliable, objective assessments of sample integrity, concentration, and fragment size. Explore the complete portfolio of automated electrophoresis instruments, application-specific assays, intuitive software, and expert technical services that provide flexible solutions for robust and objective sample quality assessment.
DNA fragment sizing with the Agilent ZAG DNA Analyzer System
The Agilent ZAG DNA Analyzer system is a parallel capillary electrophoresis system for high-throughput sizing of DNA fragments. Ideal for PCR amplicons and other DNA fragments, there are four ZAG dsDNA kits utilized on the ZAG DNA Analyzer system that cover a sizing range from 35 to 20,000 bp. Common applications of the ZAG dsDNA kits include analysis of PCR amplicons and restriction digests, genotyping, and microsatellite analysis. The ZAG DNA Analyzer system analyzes an entire 96-well plate at one time. The instrument holds nine 96-well plates that can be set up in the instrument to run continuously. Total separation times for the kits range from 30 minutes on the ZAG 135 dsDNA kit to one hour on the ZAG 105 dsDNA kit for 96 samples. In this application note, Agilent Technologies focuses on the performance of the ZAG DNA Analyzer system with the four available kits: ZAG 105 dsDNA kit (1 - 500), ZAG 110 dsDNA kit (35 - 5000 bp), ZAG 130 dsDNA kit (75 - 20000 bp), and ZAG 135 dsDNA kit (1 - 1500 bp). Fragment analysis on each kit explores the sensitivity, accuracy, and precision of sizing. Comparison of the sizing of the fragments between the kits was performed.
