Member since: 2014
Organization: Aurelia Bioscience
Great instrument, easy to use, quick to run and highly quantitative
Application Area: Western Blotting to examine the expression of proteins in cells, really good for PROTAC
"Any scientist who runs Western Blots will know that the process is technically difficult, time-consuming, and requires 'good' antibodies that have very little cross-reactivity. The WES system from Protein Simple is an automated instrument that uses glass rods/tubes to form a column, then run the protein lysate to separate proteins based on molecular weight. In any one run either 12 or 24 lanes can be run, each lane contains your lysate plus fluorescence molecular weight markers that represent controls in each lane. It is also possible to multiplex the detection of more than one protein in each lane, allowing for a loading control in the same lane from the same sample. We have been using WES for over three years to detect proteins from recombinant cells, primary cells, etc. The system is very easy to use, allows a complete run to be achieved and analyzed in 3 hours and, although it is not as cheap as 'old fashioned' Western blotting, the data is highly quantitative and completely different from the traditional approach. Aurelia would highly recommend the WES."
Member since: 2019
Application Area:Western analysis
"Finally an instrument for quick, reproducible and quantitative protein expression analysis. Western analysis entered a new era."
Member since: 2017
Organization: Mission Therapeutic Ltd
Brilliant advances in the automation of Western Blot
Application Area:Automated Western Blot
"Wes technology has helped remove the laboursome process of traditional Western Blot. You will need some time to validate your work on the technology and that requires optimisation of samples and antibodies but once that is achieved it’s a straightforward process with data generated in less than 3 hours. No more transfers, and goodbye to buffers spilled all over the work bench."
Member since: 2004
Organization: Merck Research Laboratories
Application Area:Wes 25 capillary western blot Protein Simple
"This newest automated western blot from Protein Simple is simply better than its earlier version Simon. It is 10x more sensitive and double the throughput from 12 versus to 25. Plus the run time is faster and easy to use. Everyone should get this Wes if you run regular western constantly, it will save you time."
Proteins are complex, but protein characterization shouldn't be. Are you struggling with methods that aren't quantitative? Are you spending all your precious sample just to optimize your assay? Are you waiting days to get your data? Wes™ lets you separate and analyze proteins by size from 2-440 kDa. He also gets you down to picogram-level sensitivity with just 3 µL of starting material. Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don't wait to discover, get going today with Wes!