Member since: 2020
Organization: University of Surrey
A game-changer in immunohistochemistry-based research of tumor microenvironments.
Application Area: Analysis of immune markers in tissue - multiplex IHC
"Having the Vectra Polaris in our lab has transformed our ability to analyze and understand the tumor immune microenvironment. Within a year of having this technology we have developed novel 9-plex immune marker panels and applied them to endometrial, bladder and esophageal cancer tissues. PhD students with little to no experience of IHC have quickly mastered the ability to perform multiplex IHC and are also now fully competent at using the software needed to analyze their samples. Before obtaining this technology, we were unable to carry out equivalent immunohistochemistry analyses, both in terms of multiplex staining capability and also quantitative regional analysis of multiplex-stained tissue sections. The increased information and thus understanding of the tumor microenvironments this technology is revealing to us will greatly help inform us in our development of more effective combination immunotherapies. "
Providing unparalleled speed, performance, and versatility, Vectra Polaris Automated Quantitative Pathology Imaging System goes beyond conventional brightfield and fluorescence whole-slide scanning – it integrates the power of multispectral imaging in a simplified workflow.
As part of our Phenoptics™ 2.0 translational solution, multispectral imaging on the Vectra Polaris can now be applied across a whole slide (using the 7 Color Opal Polaris reagent kit) with our state-of-the-art MOTiF™ technology. This enables the biology to be explored at multiple scales, from cell-to-cell interactions to the macroscopic tissue architecture.
The whole slide multispectral imaging capabilities creates a simpler and more robust workflow as fields of view do not need to be selected eliminating selection bias. You also retain a whole slide record, no re-scans required, so that you can easily re-analyze imagery as new understanding emerges.
With the Vectra Polaris, you can also generate unmixed annotated regions of interest up to 9 colors using our renowned liquid crystal tunable filter.
Our proprietary multispectral imaging technology coupled with the proven power of inForm® image analysis software and our Opal™ fluorophores, enables the identification and quantification of multiple biomarkers without the interference of autofluorescence as the signals are unmixed from one another. That means you can have confidence in accurately quantifying cell-to-cell interactions that are occurring, in situ, across the whole slide, in your FFPE samples.